Más A, Campión J, Aller P, Calle C
Departamento de Bioquímica y Biología Molecular, Facultad de Medicina, Universidad Complutense, Madrid, Spain.
Horm Metab Res. 1998 Mar;30(3):118-22. doi: 10.1055/s-2007-978848.
The administration of 10(-5) M epinephrine transiently decreased insulin receptor (IR) mRNA levels in U-937 human promonocytic cells, which reached their minimum value after 24 hours. Such a decrease seems to be due, at least in part, to a reduction in transcript stability, since the IR mRNA half-life was observed to decline from approximately 4h in untreated cells to 3 h in epinephrine-treated cells. Computer inspection of the sequence of the 3' untranslated region of the human IR mRNA showed nine AUUUA pentamers and three U-rich regions. These domains could be targets for a RNA-binding protein induced by treatment with epinephrine producing a destabilization of IR mRNA in U-937 cells.
给予10(-5)M肾上腺素可使U-937人原单核细胞中的胰岛素受体(IR)mRNA水平短暂下降，24小时后降至最低值。这种下降似乎至少部分是由于转录本稳定性降低所致，因为观察到IR mRNA的半衰期从未经处理的细胞中的约4小时降至肾上腺素处理的细胞中的3小时。对人IR mRNA 3'非翻译区序列的计算机检查显示有九个AUUUA五聚体和三个富含U的区域。这些结构域可能是肾上腺素处理诱导产生的一种RNA结合蛋白的作用靶点，该蛋白会使U-937细胞中的IR mRNA不稳定。
