• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Targeting of protein kinase Calpha to caveolae.

作者信息

Mineo C, Ying Y S, Chapline C, Jaken S, Anderson R G

机构信息

Department of Cell Biology and Neuroscience, University of Texas Southwestern Medical Center, Dallas, Texas 75235-9039, USA.

出版信息

J Cell Biol. 1998 May 4;141(3):601-10. doi: 10.1083/jcb.141.3.601.

DOI:10.1083/jcb.141.3.601
PMID:9566962
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2132740/
Abstract

Previously, we showed caveolae contain a population of protein kinase Calpha (PKCalpha) that appears to regulate membrane invagination. We now report that multiple PKC isoenzymes are enriched in caveolae of unstimulated fibroblasts. To understand the mechanism of PKC targeting, we prepared caveolae lacking PKCalpha and measured the interaction of recombinant PKCalpha with these membranes. PKCalpha bound with high affinity and specificity to caveolae membranes. Binding was calcium dependent, did not require the addition of factors that activate the enzyme, and involved the regulatory domain of the molecule. A 68-kD PKCalpha-binding protein identified as sdr (serum deprivation response) was isolated by interaction cloning and localized to caveolae. Antibodies against sdr inhibited PKCalpha binding. A 100-amino acid sequence from the middle of sdr competitively blocked PKCalpha binding while flanking sequences were inactive. Caveolae appear to be a membrane site where PKC enzymes are organized to carry out essential regulatory functions as well as to modulate signal transduction at the cell surface.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efe6/2132740/3e391fba666f/JCB29449.f9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efe6/2132740/18f99a854310/JCB29449.f1ac.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efe6/2132740/75c5bceb90b3/JCB29449.f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efe6/2132740/31c9810fa311/JCB29449.f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efe6/2132740/855da89f5230/JCB29449.f4c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efe6/2132740/46c5ee5756ca/JCB29449.f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efe6/2132740/89c5094b9abf/JCB29449.f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efe6/2132740/a9f53ffca790/JCB29449.f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efe6/2132740/2cab136dbf2a/JCB29449.f8ad.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efe6/2132740/3e391fba666f/JCB29449.f9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efe6/2132740/18f99a854310/JCB29449.f1ac.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efe6/2132740/75c5bceb90b3/JCB29449.f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efe6/2132740/31c9810fa311/JCB29449.f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efe6/2132740/855da89f5230/JCB29449.f4c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efe6/2132740/46c5ee5756ca/JCB29449.f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efe6/2132740/89c5094b9abf/JCB29449.f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efe6/2132740/a9f53ffca790/JCB29449.f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efe6/2132740/2cab136dbf2a/JCB29449.f8ad.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/efe6/2132740/3e391fba666f/JCB29449.f9.jpg

相似文献

1
Targeting of protein kinase Calpha to caveolae.
J Cell Biol. 1998 May 4;141(3):601-10. doi: 10.1083/jcb.141.3.601.
2
Protein kinase calpha targeting is regulated by temporal and spatial changes in intracellular free calcium concentration [Ca(2+)](i).蛋白激酶Cα的靶向作用受细胞内游离钙浓度[Ca(2+)]i的时空变化调控。
FASEB J. 2000 Aug;14(11):1653-63. doi: 10.1096/fj.14.11.1653.
3
Activated protein kinase C isoforms target to cardiomyocyte caveolae : stimulation of local protein phosphorylation.活化的蛋白激酶C亚型定位于心肌细胞小窝：刺激局部蛋白磷酸化。
Circ Res. 1999 May 14;84(9):980-8. doi: 10.1161/01.res.84.9.980.
4
Escherichia coli K1 internalization via caveolae requires caveolin-1 and protein kinase Calpha interaction in human brain microvascular endothelial cells.通过小窝蛋白介导的大肠杆菌K1内化需要人脑微血管内皮细胞中的小窝蛋白-1与蛋白激酶Cα相互作用。
J Biol Chem. 2002 Dec 27;277(52):50716-24. doi: 10.1074/jbc.M208830200. Epub 2002 Oct 16.
5
Additional binding sites for anionic phospholipids and calcium ions in the crystal structures of complexes of the C2 domain of protein kinase calpha.蛋白激酶Cα的C2结构域复合物晶体结构中阴离子磷脂和钙离子的额外结合位点。
J Mol Biol. 2002 Jul 5;320(2):277-91. doi: 10.1016/S0022-2836(02)00464-3.
6
Phospholipase D1 in caveolae: regulation by protein kinase Calpha and caveolin-1.小窝中的磷脂酶D1：由蛋白激酶Cα和小窝蛋白-1调控。
Biochemistry. 1999 Mar 23;38(12):3763-9. doi: 10.1021/bi982478+.
7
Mechanisms of regulation of phospholipase D1 by protein kinase Calpha.蛋白激酶Cα对磷脂酶D1的调控机制
J Biol Chem. 2003 Jan 24;278(4):2348-55. doi: 10.1074/jbc.M210093200. Epub 2002 Nov 12.
8
Analysis of the subcellular distribution of protein kinase Calpha using PKC-GFP fusion proteins.
Exp Cell Res. 2000 Jul 10;258(1):204-14. doi: 10.1006/excr.2000.4925.
9
Inhibitory properties of the regulatory domains of human protein kinase Calpha and mouse protein kinase Cepsilon.人蛋白激酶Cα和小鼠蛋白激酶Cε调节结构域的抑制特性。
J Biol Chem. 1998 Apr 10;273(15):8940-5. doi: 10.1074/jbc.273.15.8940.
10
Determination of the calcium-binding sites of the C2 domain of protein kinase Calpha that are critical for its translocation to the plasma membrane.确定蛋白激酶Cα的C2结构域中对其向质膜转位至关重要的钙结合位点。
Biochem J. 1999 Feb 1;337 ( Pt 3)(Pt 3):513-21.

引用本文的文献

1
Calcium Signaling Dynamics in Vascular Cells and Their Dysregulation in Vascular Disease.血管细胞中的钙信号动力学及其在血管疾病中的失调
Biomolecules. 2025 Jun 18;15(6):892. doi: 10.3390/biom15060892.
2
Mechanical forces pattern endocardial Notch activation via mTORC2-PKC pathway.机械力通过mTORC2-PKC途径调控心内膜Notch激活模式。
Elife. 2025 Feb 11;13:RP97268. doi: 10.7554/eLife.97268.
3
CAVIN-2 positively correlates with diabetic PAD and promotes LDL transcytosis by inhibiting eNOS activation.CAVIN-2与糖尿病性外周动脉疾病呈正相关，并通过抑制内皮型一氧化氮合酶（eNOS）的激活促进低密度脂蛋白（LDL）的转胞吞作用。

本文引用的文献

1
Caveolae, caveolin and caveolin-rich membrane domains: a signalling hypothesis.小窝、小窝蛋白及富含小窝蛋白的膜结构域：一种信号传导假说。
Trends Cell Biol. 1994 Jul;4(7):231-5. doi: 10.1016/0962-8924(94)90114-7.
2
The caveolae membrane system.小窝膜系统。
Annu Rev Biochem. 1998;67:199-225. doi: 10.1146/annurev.biochem.67.1.199.
3
Caveolin interaction with protein kinase C. Isoenzyme-dependent regulation of kinase activity by the caveolin scaffolding domain peptide.小窝蛋白与蛋白激酶C的相互作用。小窝蛋白支架结构域肽对激酶活性的同工酶依赖性调节。
Ann Med. 2025 Dec;57(1):2457526. doi: 10.1080/07853890.2025.2457526. Epub 2025 Jan 31.
4
Spatiotemporal control of kinases and the biomolecular tools to trace activity.激酶的时空控制以及用于追踪活性的生物分子工具。
J Biol Chem. 2024 Nov;300(11):107846. doi: 10.1016/j.jbc.2024.107846. Epub 2024 Oct 1.
5
G protein-coupled estrogen receptor (GPER) in the dorsal hippocampus regulates memory consolidation in gonadectomized male mice, likely via different signaling mechanisms than in female mice.背侧海马中的 G 蛋白偶联雌激素受体 (GPER) 在去势雄性小鼠中调节记忆巩固，其可能通过与雌性小鼠不同的信号机制来实现。
Horm Behav. 2024 May;161:105516. doi: 10.1016/j.yhbeh.2024.105516. Epub 2024 Mar 1.
6
Unraveling the Cave: A Seventy-Year Journey into the Caveolar Network, Cellular Signaling, and Human Disease.揭开洞穴的奥秘：七十载探索陷窝网络、细胞信号转导与人类疾病
Cells. 2023 Nov 22;12(23):2680. doi: 10.3390/cells12232680.
7
Opioid-Modulated Receptor Localization and Erk1/2 Phosphorylation in Cells Coexpressing μ-Opioid and Nociceptin Receptors.在共表达 μ 阿片受体和孤啡肽受体的细胞中，阿片调制受体定位和 Erk1/2 磷酸化。
Int J Mol Sci. 2023 Jan 5;24(2):1048. doi: 10.3390/ijms24021048.
8
Integrated Bioinformatic Analysis of the Expression and Prognosis of Caveolae-Related Genes in Human Breast Cancer.人乳腺癌中小窝相关基因表达与预后的综合生物信息学分析
Front Oncol. 2021 Aug 26;11:703501. doi: 10.3389/fonc.2021.703501. eCollection 2021.
9
Endothelial pannexin 1-TRPV4 channel signaling lowers pulmonary arterial pressure in mice.内皮细胞 Pannexin 1-TRPV4 通道信号降低小鼠肺动脉压。
Elife. 2021 Sep 7;10:e67777. doi: 10.7554/eLife.67777.
10
Lessons from cavin-1 deficiency.Cavin-1 缺乏的启示。
Biochem Soc Trans. 2020 Feb 28;48(1):147-154. doi: 10.1042/BST20190380.
J Biol Chem. 1997 Dec 26;272(52):33416-21. doi: 10.1074/jbc.272.52.33416.
4
Structure of detergent-resistant membrane domains: does phase separation occur in biological membranes?抗去污剂膜结构域的结构：生物膜中会发生相分离吗？
Biochem Biophys Res Commun. 1997 Nov 7;240(1):1-7. doi: 10.1006/bbrc.1997.7575.
5
Physical association with ras enhances activation of membrane-bound raf (RafCAAX).与Ras的物理结合增强了膜结合型Raf（RafCAAX）的激活。
J Biol Chem. 1997 Apr 18;272(16):10345-8. doi: 10.1074/jbc.272.16.10345.
6
A protein kinase Cdelta-binding protein SRBC whose expression is induced by serum starvation.一种蛋白激酶Cδ结合蛋白SRBC，其表达由血清饥饿诱导。
J Biol Chem. 1997 Mar 14;272(11):7381-9. doi: 10.1074/jbc.272.11.7381.
7
Protein kinase C: ports of anchor in the cell.
Curr Biol. 1996 Jul 1;6(7):806-9. doi: 10.1016/s0960-9822(02)00600-0.
8
Protein kinase C isozymes and substrates.蛋白激酶C同工酶与底物。
Curr Opin Cell Biol. 1996 Apr;8(2):168-73. doi: 10.1016/s0955-0674(96)80062-7.
9
Localization of epidermal growth factor-stimulated Ras/Raf-1 interaction to caveolae membrane.表皮生长因子刺激的Ras/Raf-1相互作用在小窝膜上的定位。
J Biol Chem. 1996 May 17;271(20):11930-5. doi: 10.1074/jbc.271.20.11930.
10
Coordination of three signaling enzymes by AKAP79, a mammalian scaffold protein.哺乳动物支架蛋白AKAP79对三种信号酶的协调作用。
Science. 1996 Mar 15;271(5255):1589-92. doi: 10.1126/science.271.5255.1589.