Kavun E M, Kolibo D V, Borisova E G
Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kiev, Ukraine.
Prikl Biokhim Mikrobiol. 1998 Mar-Apr;34(2):214-9.
Structural analysis of the B-epitopes formed on protein macromolecules by glutaraldehyde treatment with subsequent block of free active groups by glycine was performed. Two types of antigenic determinants recognized by different antibody populations were found by protein chemistry and immunochemistry techniques. An efficient method of blocking the antibodies to the epitopes formed on proteins by glutaraldehyde was developed.
对经戊二醛处理后用甘氨酸封闭游离活性基团的蛋白质大分子上形成的B表位进行了结构分析。通过蛋白质化学和免疫化学技术发现了不同抗体群体识别的两种抗原决定簇。开发了一种有效封闭戊二醛在蛋白质上形成的表位抗体的方法。
