髓鞘碱性蛋白之间的免疫化学比较。

Immunochemical comparisons among myelin basic proteins.

Whitaker J N

Research and Neurology Services, Memphis Veterans Hospital, TN 38104.

Comp Biochem Physiol B. 1978;59(4):299-306. doi: 10.1016/0305-0491(78)90006-8.

Abstract

The relationships among myelin encephalitogenic or basic proteins were immunochemically examined. 2. Rabbit antisera to myelin basic proteins isolated from chicken, rabbit, bovine, guinea-pig, and human brain specimens were prepared. By quantitative microcomplement fixation these rabbit antisera were used to measure cross-reactions among the myelin basic proteins of the turtle, chicken, rat, rabbit, cow, pig, sheep, dog, guinea-pig, monkey and human. 3. A spectrum of cross-reactivities was detected even though some cross-reactivity persisted across major phylogenetic barriers. Varying, but sometimes marked, differences existed in reactivities of the small and large basic proteins of the rat. Reciprocity of cross-reactivities among basic proteins was inconstant. 4. This study demonstrates the range of immunochemical cross-reactivities among myelin basic proteins and the sensitivity of quantitative microcomplement fixation in assessing such antigenic or conformational differences.

摘要

对髓鞘致脑炎蛋白或碱性蛋白之间的关系进行了免疫化学检测。2. 制备了针对从鸡、兔、牛、豚鼠和人脑标本中分离出的髓鞘碱性蛋白的兔抗血清。通过定量微量补体结合试验，使用这些兔抗血清来测定龟、鸡、大鼠、兔、牛、猪、羊、狗、豚鼠、猴和人的髓鞘碱性蛋白之间的交叉反应。3. 尽管一些交叉反应在主要的系统发育障碍中持续存在，但仍检测到了一系列交叉反应性。大鼠的小碱性蛋白和大碱性蛋白的反应性存在差异，有时差异明显。碱性蛋白之间交叉反应的相互性并不恒定。4. 本研究证明了髓鞘碱性蛋白之间免疫化学交叉反应的范围以及定量微量补体结合试验在评估此类抗原或构象差异方面的敏感性。