Naidu R, Yadav M, Nair S, Kutty K K
Department of Genetics and Cellular Biology, University of Malaya, Kuala Lumpur.
Anticancer Res. 1998 Jan-Feb;18(1A):65-70.
Expression of p53 protein was investigated by immunohistochemical techniques in archival cases of 134 primary breast carcinomas comprising 13 comedo ductal carcinoma in situ (DCIS), 105 invasive ductal carcinomas, 7 contained the comedo DCIS component adjacent to the invasive ductal component, 5 invasive lobular carcinomas, three colloid carcinomas and one medullary carcinoma. Overexpression of p53 gene product was studied to determine the association with clinico-pathological parameters and also its relationship to c-erbB2. Overexpression of p53 protein was observed in 31% (4/13) of comedo DCIS, 37% (39/105) of invasive ductal carcinomas, 57% (4/7) of carcinomas containing both the in situ and invasive lesions and all medullary carcinomas. A significant relationship (p < 0.05) was observed between strong immunoreactivity of p53 protein and absence of estrogen receptor, histological grade and c-erbB2 but not with lymph node metastases or age of patient. These observations suggest that overexpression of p53 protein may play an important role in tumor progression from noninvasive to invasive in some breast carcinomas and may have potential as an indicator for poorer prognosis.
采用免疫组化技术对134例原发性乳腺癌存档病例进行p53蛋白表达研究，其中包括13例粉刺型导管原位癌（DCIS）、105例浸润性导管癌、7例在浸润性导管成分旁含有粉刺型DCIS成分、5例浸润性小叶癌、3例黏液癌和1例髓样癌。研究p53基因产物的过表达情况，以确定其与临床病理参数的相关性以及与c-erbB2的关系。在粉刺型DCIS中，31%（4/13）观察到p53蛋白过表达；在浸润性导管癌中，37%（39/105）观察到过表达；在同时含有原位和浸润性病变的癌中，57%（4/7）观察到过表达；在所有髓样癌中均观察到过表达。p53蛋白强免疫反应性与雌激素受体缺失、组织学分级和c-erbB2之间存在显著相关性（p<0.05），但与淋巴结转移或患者年龄无关。这些观察结果表明，p53蛋白过表达可能在某些乳腺癌从非侵袭性向侵袭性的肿瘤进展中起重要作用，并且可能具有作为预后较差指标的潜力。
