Yoshida Y, Tasaki K, Kimurai M, Takenaga K, Yamamoto H, Yamaguchi T, Saisho H, Sakiyama S, Tagawa M
Division of Pathology, Chiba Cancer Center Research Institute, Japan.
Anticancer Res. 1998 Jan-Feb;18(1A):333-5.
We have examined antitumor effect of human pancreatic carcinoma cells (AsPC-1) retrovirally transduced with interleukin-12 (IL-12), IL-15 or IL-18 gene in nude mice. The tumor growth of IL-12-expressing AsPC-1 cells was significantly retarded and that of IL-15-expressing cells was also impeded compared with that of wild-type cells, although their in vitro cell growth remained unchanged. However, the expression of IL-18 in AsPC-1 cells did not generate any antitumor effect since the tumor growth of the transduced cells was the same as that of wild-type cells. Thus, the differential actions of these cytokines on non-T cells can generate a variety of antitumor effect in nude mice, although their actions on T cells lineage favor the stimulation of Th1-type helper T cells.
我们检测了用白细胞介素-12（IL-12）、IL-15或IL-18基因逆转录病毒转导的人胰腺癌细胞（AsPC-1）在裸鼠中的抗肿瘤作用。与野生型细胞相比，表达IL-12的AsPC-1细胞的肿瘤生长明显延迟，表达IL-15的细胞的肿瘤生长也受到抑制，尽管它们的体外细胞生长没有变化。然而，AsPC-1细胞中IL-18的表达没有产生任何抗肿瘤作用，因为转导细胞的肿瘤生长与野生型细胞相同。因此，这些细胞因子对非T细胞的不同作用可在裸鼠中产生多种抗肿瘤作用，尽管它们对T细胞谱系的作用有利于刺激Th1型辅助性T细胞。
