Kindas-Mügge I, Micksche M, Trautinger F
Institute of Tumor Biology-Cancer Research, University of Vienna, Austria. ingela-margaret.kindas-muegge@,univie.ac.at.
Anticancer Res. 1998 Jan-Feb;18(1A):413-7.
The role of hsp27 in the regulation of cell growth has been investigated in the breast cancer cell line MDA-MB-23 1. Cells were co-transfected with an expression vector carrying the human hsp27 gene (pSG-2711) and a plasmid conferring neomycin resistance (pWlneo). Transfected cells were selected for neomycin resistance. Stable transfectants were used as a pooled population for further experiments, since single-cell colonies were not able to grow into mass culture under continuous selection pressure. Over-expression of hsp27 was analysed by immunohistochemistry and immunoblotting. Cells only transfected for neomycin were used as control cells. The growth rate of the transfected cell line was determined whether overexpression of hsp27 directly influences the growth properties of the cells. Growth analysis of transfected cell lines in vitro revealed a lower proliferation rate of the hsp27 overexpressing cells compared to controls. These data suggest that hsp27 is involved in downregulation of cell proliferation in this human breast cancer cell line.
热休克蛋白27（hsp27）在细胞生长调控中的作用已在乳腺癌细胞系MDA-MB-231中进行了研究。将携带人hsp27基因的表达载体（pSG-2711）与赋予新霉素抗性的质粒（pWlneo）共转染细胞。选择对新霉素具有抗性的转染细胞。由于单细胞集落在持续选择压力下无法生长成大规模培养物，因此将稳定转染子作为混合群体用于进一步实验。通过免疫组织化学和免疫印迹分析hsp27的过表达。仅转染新霉素的细胞用作对照细胞。确定转染细胞系的生长速率，以了解hsp27的过表达是否直接影响细胞的生长特性。体外转染细胞系的生长分析显示，与对照相比，hsp27过表达细胞的增殖速率较低。这些数据表明，hsp27参与了该人乳腺癌细胞系中细胞增殖的下调。
