利用免疫细胞化学和共聚焦激光扫描显微镜对p53过表达进行细胞学应用。
Cytologic application of p53 overexpression using immunocytochemistry and confocal laser scanning microscopy.
作者信息
Sato S, Ito K, Jiko K, Konno R, Yajima A, Sasano H
机构信息
Department of Obstetrics and Gynecology, Tohoku University School of Medicine, Sendai, Japan.
出版信息
Acta Cytol. 1998 Mar-Apr;42(2):342-5. doi: 10.1159/000331613.
OBJECTIVE
p53 Protein plays an important role in cellular growth control. This study investigated p53 protein expression in cell smears of endometrial carcinomas supplemented by confocal laser scanning microscopy (CLSM).
STUDY DESIGN
Imprints from surgical specimens of 20 endometrial carcinomas were used. p53 Protein expression was investigated immunocytochemically using the monoclonal antibody pAb1801. Using CLSM, three-dimensional morphology was studied.
RESULTS
Of the 20 cases of endometrial carcinoma, 8 stained positively for p53 protein. p53 Showed heterogeneous intranuclear localization, which appeared to be associated with chromatin structure.
CONCLUSION
Immunocytochemical detection of p53 overexpression in cell samples is practical, and CLSM has vast potentials in studying the intranuclear arrangement of chromatin.
目的
p53蛋白在细胞生长控制中起重要作用。本研究通过共聚焦激光扫描显微镜（CLSM）对子宫内膜癌的细胞涂片进行p53蛋白表达研究。
研究设计
使用20例子宫内膜癌手术标本的印片。采用单克隆抗体pAb1801进行免疫细胞化学研究p53蛋白表达。利用CLSM研究三维形态。
结果
20例子宫内膜癌中，8例p53蛋白染色呈阳性。p53显示出异质性核内定位，这似乎与染色质结构有关。
结论
免疫细胞化学检测细胞样本中p53过表达是可行的，CLSM在研究染色质核内排列方面具有巨大潜力。
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