Lin L I, Lee P H, Wu C M, Lin J K
School of Medical Technology, College of Medicine, National Taiwan University, Taipei, Taiwan.
Anticancer Res. 1998 Jan-Feb;18(1B):541-6.
It has been proposed that nm23-H1, a candidate suppressor gene for metastasis, plays an important role in the metastasis of human tumors. In order to investigate its role in the human hepatocellular carcinoma (HCC), 18 matched pairs of tumorous and adjacent nontumorous liver tissues of hepatectomy from patients with HCC were studied by quantitative reverse transcription-PCR. Further analyses of the nm23-H1 gene were also done. The results of these molecular studies were correlated with the clinicopathologic features of the patients. Nm23-H1 transcript was expressed in all neoplastic and adjacent nontumorous liver tissue. The level of expression, however, did not correlate well with the extension or metastatic potential of the tumors. Instead, in 15 (83.3%) of 18 HCC, nm23-H1 expression was higher in the tumorous tissues, compared with the adjacent nontumorous tissues; and significantly higher levels of nm23-H1 mRNA expression was detected in HCC with poor differentiation (Edmonson classification, III and IV) than those with moderate differentiation (I and II). Southern blot analysis of nm23-H1 gene revealed neither amplification nor loss of heterozygosity of all HCC tissues examined. Direct sequencing of the nm23-H1 gene in all HCC tissues detected no mutations. Our findings suggested that increased nm23-H1 mRNA expression is correlated with HCC tumor progression.
有人提出，nm23-H1作为一种转移候选抑制基因，在人类肿瘤转移中发挥重要作用。为了研究其在人类肝细胞癌（HCC）中的作用，我们采用定量逆转录-聚合酶链反应（PCR）对18例HCC患者肝切除术中肿瘤及相邻非肿瘤肝组织的配对样本进行了研究。同时，对nm23-H1基因进行了进一步分析。这些分子研究结果与患者的临床病理特征相关联。nm23-H1转录本在所有肿瘤及相邻非肿瘤肝组织中均有表达。然而，其表达水平与肿瘤的扩展或转移潜能并无良好的相关性。相反，在18例HCC中的15例（83.3%）中，与相邻非肿瘤组织相比，肿瘤组织中nm23-H1表达更高；与中度分化（I级和II级）的HCC相比，低分化（Edmonson分级，III级和IV级）的HCC中nm23-H1 mRNA表达水平显著更高。对nm23-H1基因的Southern印迹分析显示，所有检测的HCC组织均未出现扩增或杂合性缺失。对所有HCC组织中nm23-H1基因的直接测序未检测到突变。我们的研究结果表明，nm23-H1 mRNA表达增加与HCC肿瘤进展相关。
