Iizuka N, Oka M, Noma T, Nakazawa A, Hirose K, Suzuki T
Department of Surgery II, Yamaguchi University School of Medicine, Japan.
Cancer Res. 1995 Feb 1;55(3):652-7.
nm23 was originally identified as an antimetastatic gene, the expression of which was inversely correlated with tumor metastatic potential in rodent model systems. Subsequently, two related human nm23 genes, nm23-H1 and nm23-H2, were identified. The relationship between expression of nm23-H1 and nm23-H2 in hepatocellular carcinoma specimens from 30 patients and metastatic potential was investigated with the use of a quantitative reverse transcription-PCR procedure. The abundance of nm23-H1 and nm23-H2 mRNA was compared with serum alpha-fetoprotein concentration, tumor size (maximum diameter), and histopathological parameters such as portal vein tumor thrombus, intrahepatic metastasis, capsular formation, capsular infiltration, differentiation of tumor cells, and TNM stage. The abundance of nm23-H1 mRNA showed a significant inverse correlation with intrahepatic metastasis and TNM stage. Furthermore, we confirmed that reduced expression of nm23-H1 mRNA was in accordance with a reduced amount of NM23-H1 protein using Western blot analysis. No correlation was apparent between nm23-H2 mRNA abundance and intrahepatic metastasis. These data support the conclusion that nm23-H1 may play a more important role than nm23-H2 in intrahepatic metastasis in hepatocellular carcinoma. Furthermore, nm23-H1 mRNA abundance may be a predictor of intrahepatic metastasis, the most important factor correlated with the metastatic potential of hepatocellular carcinoma.
nm23最初被鉴定为一种抗转移基因，在啮齿动物模型系统中，其表达与肿瘤转移潜能呈负相关。随后，鉴定出了两个相关的人类nm23基因，即nm23-H1和nm23-H2。采用定量逆转录-聚合酶链反应方法，研究了30例肝细胞癌标本中nm23-H1和nm23-H2的表达与转移潜能之间的关系。将nm23-H1和nm23-H2 mRNA的丰度与血清甲胎蛋白浓度、肿瘤大小（最大直径）以及组织病理学参数（如门静脉癌栓、肝内转移、包膜形成、包膜浸润、肿瘤细胞分化和TNM分期）进行了比较。nm23-H1 mRNA的丰度与肝内转移和TNM分期呈显著负相关。此外，我们通过蛋白质印迹分析证实，nm23-H1 mRNA表达降低与NM23-H1蛋白量减少一致。nm23-H2 mRNA丰度与肝内转移之间无明显相关性。这些数据支持以下结论：nm23-H1在肝细胞癌肝内转移中可能比nm23-H
