Zenteno-Savin T, Castellini M A
Institute of Marine Science, University of Alaska Fairbanks, USA.
Comp Biochem Physiol C Pharmacol Toxicol Endocrinol. 1998 Jan;119(1):1-6. doi: 10.1016/s0742-8413(97)00136-9.
We used radioimmunoassay methods to quantify arginine vasopressin (AVP), atrial natriuretic peptide (ANP), and angiotensin II (Ang II) in plasma samples from harbor seals (Phoca vitulina richardsii), Weddell seals (Leptonychotes weddellii), northern elephant seals (Mirounga angustirostris), ringed seals (Phoca hispida), California sea lions (Zalophus californianus), and Steller sea lions (Eumetopias jubatus). Plasma concentrations of AVP, ANP, and Ang II in these pinniped species were within the ranges reported for other vertebrates under resting conditions. However, there were species, geographic and developmental variations in these hormones: Levels of AVP in plasma samples from adult Steller sea lions and harbor seals were higher than in pups of the same species; higher levels of plasma ANP were found in wild captured Alaskan Steller sea lions and in hunted ringed seals; differences in plasma levels of all three hormones were found throughout the geographic distribution of harbor seals and Steller sea lions in Alaska. This is the first report on circulating concentrations of vasoactive hormones in pinnipeds, and demonstrates that further studies are needed to ascertain the natural variability in these levels with the impact of molting, fasting, diving and environmental factors in seals and sea lions.
我们使用放射免疫分析法对斑海豹（Phoca vitulina richardsii）、威德尔海豹（Leptonychotes weddellii）、北海象海豹（Mirounga angustirostris）、环斑海豹（Phoca hispida）、加利福尼亚海狮（Zalophus californianus）和北海狗（Eumetopias jubatus）血浆样本中的精氨酸加压素（AVP）、心房利钠肽（ANP）和血管紧张素II（Ang II）进行定量分析。这些鳍脚类动物血浆中AVP、ANP和Ang II的浓度处于其他脊椎动物在静息状态下所报道的范围内。然而，这些激素存在物种、地理和发育上的差异：成年北海狗和斑海豹血浆样本中的AVP水平高于同物种幼崽；在野生捕获的阿拉斯加北海狗和被捕杀的环斑海豹中发现血浆ANP水平较高；在阿拉斯加斑海豹和北海狗的整个地理分布范围内，发现这三种激素的血浆水平存在差异。这是关于鳍脚类动物血管活性激素循环浓度的首次报告，并表明需要进一步研究以确定这些水平的自然变异性以及海豹和海狮蜕皮、禁食、潜水和环境因素的影响。
