Viana da Costa A, Gaubert S, Fontaine J, Lafitte S, Seixas A, De Lourdes Sampaio Silva M, Capron A, Grzych J M
Centre d'immunologie et de Biologie Parasitaire, Unité INSERM U-167, Institut Pasteur de Lille, France.
Parasite Immunol. 1998 Mar;20(3):143-53. doi: 10.1046/j.1365-3024.1998.00136.x.
Humoral and cellular responses to Schistosoma bovis antigens have been evaluated over a period of 11 weeks in mice exposed to S. bovis cercariae and data analysed in the context of the parasitic parameters (worm and egg loads) recorded at days 30, 60 and 80 of the ongoing infection. Results revealed a decrease of worm burden, particularly marked for female worms, between day 60 and day 80 of infection suggesting a higher susceptibility of female schistosomes to attrition mechanisms. The B-cell response, studied by measuring the production of different isotypes, was directed against different stage specific antigens, with a predominance of IgG1 antibodies associated with a significant increase of IgA and IgE antibodies after egg deposition. The T-cell response, assessed after in vitro stimulation of splenocytes, showed a predominant production of Th-2 cytokines (IL-4, IL-5 and IL-10) occurring after egg laying. Interestingly in contrast to S. mansoni infection the Th-2 polarization did not seem to be exclusively triggered by egg-associated antigens since significant amounts of IL-10 were produced after stimulation with adult worm antigen preparation (SWAP) before the beginning of egg deposition.
在感染牛血吸虫尾蚴的小鼠中，对牛血吸虫抗原的体液和细胞反应已在11周的时间内进行了评估，并结合在持续感染的第30、60和80天记录的寄生虫参数（虫体和虫卵负荷）对数据进行了分析。结果显示，在感染的第60天至第80天之间，虫体负担有所减轻，尤其是雌虫，这表明雌性血吸虫对损耗机制的敏感性更高。通过测量不同同种型的产生来研究B细胞反应，该反应针对不同阶段的特异性抗原，其中IgG1抗体占主导，在虫卵沉积后IgA和IgE抗体显著增加。在体外刺激脾细胞后评估的T细胞反应显示，产卵后主要产生Th-2细胞因子（IL-4、IL-5和IL-10）。有趣的是，与曼氏血吸虫感染不同，Th-2极化似乎并非仅由虫卵相关抗原触发，因为在虫卵沉积开始前，用成虫抗原制剂（SWAP）刺激后会产生大量的IL-10。
