Bao L, Loda M, Zetter B R
Department of Surgery and Cell Biology, Children's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Clin Exp Metastasis. 1998 Apr;16(3):227-33. doi: 10.1023/a:1006540824969.
We previously isolated thymosin beta15 from highly metastatic Dunning rat prostatic carcinoma cells. Immunohistochemical study of human prostate cancer specimens revealed a general correlation between Gleason grade and thymosin beta15 expression, with high-grade (more malignant) tumors showing increased staining compared to low-grade tumors. To determine whether thymosin beta15 may be differentially expressed in cancer cells with different metastatic potential other than in the prostatic carcinoma cells, we examined thymosin beta15 mRNA levels in tumor cell lines from different species. We also examined thymosin beta15 protein levels in human breast cancer samples. Thymosin beta15 was upregulated in the highly metastatic mouse lung and human breast cancer cell lines in comparison to the nonmetastatic counterparts. Immunohistochemical staining showed the evidence of upregulation of thymosin beta15 in malignant human breast carcinomas as compared to benign breast tumors. The expression of thymosin beta15 was correlated with the metastatic potential of the mouse lung carcinoma and human breast carcinoma cells in addition to the prostatic carcinomas. Thymosin beta15 may be a useful marker to predict metastatic potential of certain human cancers.
我们之前从高转移性的邓宁大鼠前列腺癌细胞中分离出了胸腺素β15。对人类前列腺癌标本的免疫组织化学研究显示，格里森分级与胸腺素β15表达之间存在普遍相关性，与低级别肿瘤相比，高级别（恶性程度更高）肿瘤的染色增加。为了确定胸腺素β15是否在除前列腺癌细胞外具有不同转移潜能的癌细胞中差异表达，我们检测了来自不同物种的肿瘤细胞系中胸腺素β15的mRNA水平。我们还检测了人类乳腺癌样本中胸腺素β15的蛋白水平。与非转移性对应细胞系相比，胸腺素β15在高转移性的小鼠肺癌和人类乳腺癌细胞系中上调。免疫组织化学染色显示，与良性乳腺肿瘤相比，恶性人类乳腺癌中胸腺素β15上调。除前列腺癌外，胸腺素β15的表达与小鼠肺癌和人类乳腺癌细胞的转移潜能相关。胸腺素β15可能是预测某些人类癌症转移潜能的有用标志物。
