Chen J, Ido E, Jin M, Kuwata T, Igarashi T, Mizuno A, Koyanagi Y, Hayami M
Laboratory of Pathogenic Virus, Institute for Virus Research, Kyoto University, Japan.
J Gen Virol. 1998 Apr;79 ( Pt 4):741-5. doi: 10.1099/0022-1317-79-4-741.
To investigate the transferability of macrophage (Mphi)-tropism among primate lentiviruses, we constructed recombinants of human immunodeficiency virus type 1 (HIV-1), simian immunodeficiency virus strain mac (SIVmac) and chimeric HIV-1/SIVmac (SHIV) having env region Mphi-tropic determinants. A recombinant HIV-1 having env partially derived from a Mphi-tropic HIV-1 strain (JR-FL) replicated in human macrophages but not in monkey macrophages. Conversely, a recombinant SIVmac having env from a Mphi-tropic strain (SIVmac316) replicated in monkey macrophages but not in human macrophages. A new SHIV (designated NM-3rN/JRFL) carrying the LTR and gag, pol, vif, vpx and nef of SIVmac and vpr, tat, rev, vpu and env of HIV-1 with env partially replaced by that of JR-FL was replication-competent in human macrophages but not in monkey macrophages. These results suggest that the Mphi-tropic determinant is specific to each host species and that the mechanism of Mphi-tropism is different between HIV and SIV.
为研究巨噬细胞嗜性在灵长类慢病毒间的可转移性，我们构建了具有env区巨噬细胞嗜性决定簇的1型人类免疫缺陷病毒（HIV-1）、猕猴免疫缺陷病毒株mac（SIVmac）及嵌合HIV-1/SIVmac（SHIV）的重组体。一种env部分源自巨噬细胞嗜性HIV-1毒株（JR-FL）的重组HIV-1能在人巨噬细胞中复制，但不能在猴巨噬细胞中复制。相反，一种env来自巨噬细胞嗜性毒株（SIVmac316）的重组SIVmac能在猴巨噬细胞中复制，但不能在人巨噬细胞中复制。一种新的SHIV（命名为NM-3rN/JRFL）携带SIVmac的LTR以及gag、pol、vif、vpx和nef，HIV-1的vpr、tat、rev、vpu和env，其中env部分被JR-FL的env取代，该病毒在人巨噬细胞中具有复制能力，但在猴巨噬细胞中无此能力。这些结果表明，巨噬细胞嗜性决定簇对每个宿主物种具有特异性，且HIV和SIV之间巨噬细胞嗜性的机制不同。
