Le Feuvre C, Le Quan Sang K H, Brunet A, Catuli D, Metzger J P, Vacheron A, Devynck M A
Clinique Cardiologique, Hôpital Necker-Enfants Malades, Paris, France.
Thromb Haemost. 1998 Apr;79(4):837-42.
This study was designed to assess whether platelet Ca2+ handling or membrane microviscosity could be considered as indexes of vascular tone, or could help to predict an increased risk of restenosis after coronary angioplasty. Vascular tone was quantified in 21 patients with stable angina by the vasodilator response to sin-1 intracoronary injection in the reference coronary segment and by the importance of the acute recoil after angioplasty in the narrowed segment. The degree of restenosis was quantified by coronary angiography 6 months later. Individual values of relative sin-1-induced changes in the reference coronary diameter were positively correlated with cytosolic Ca2+ concentration in unstimulated platelets, irrespective of the extracellular Ca2+ concentration (p < 0.01). This relationship was also observed with the thrombin-evoked Ca2+ changes, measured in the absence of a Ca2+ influx (p = 0.01). No relationship was found between sin-1-induced coronary changes and membrane microviscosity evaluated by TMA-DPH and DPH anisotropies or platelet volume, or between degree of acute recoil and platelet characteristics. In conclusion, platelet Ca2+ reflects the vasodilating efficacy in response to sin-1, but cannot help to predict restenosis after coronary angioplasty.
本研究旨在评估血小板Ca2+处理或膜微粘度是否可被视为血管张力指标，或是否有助于预测冠状动脉成形术后再狭窄风险增加。通过在参考冠状动脉节段对sin-1进行冠状动脉内注射后的血管舒张反应以及血管成形术后狭窄节段急性回缩的程度，对21例稳定型心绞痛患者的血管张力进行量化。6个月后通过冠状动脉造影对再狭窄程度进行量化。无论细胞外Ca2+浓度如何，参考冠状动脉直径相对sin-1诱导变化的个体值与未刺激血小板中的胞质Ca2+浓度呈正相关（p < 0.01）。在无Ca2+内流情况下测量的凝血酶诱发的Ca2+变化也观察到这种关系（p = 0.01）。在sin-1诱导的冠状动脉变化与通过TMA-DPH和DPH各向异性评估的膜微粘度或血小板体积之间，以及急性回缩程度与血小板特征之间均未发现相关性。总之，血小板Ca2+反映了对sin-1的血管舒张功效，但无助于预测冠状动脉成形术后的再狭窄。
