硫胺素和四氢糠基二硫化硫胺素对豚鼠心房乙酰胆碱酯酶活性影响的超微细胞化学研究

Ultracytochemical studies on the effects of thiamine and thiamine tetrahydrofurfuryl disulfide on acetylcholinesterase activity in guinea pig atria.

作者信息

Ogawa K, Hirano H, Shinozaki H

出版信息

J Nutr Sci Vitaminol (Tokyo). 1976;22(2):71-8. doi: 10.3177/jnsv.22.71.

DOI:10.3177/jnsv.22.71

Abstract

As a result of histological and ultracytochemical investigations concerning the influence of thiamine and its disulfide derivative, thiamine tetrahydrofurfuryl disulfide (TTFD), on acetylcholinesterase (AChE) in extirpated guinea pig atria, it was found that there was a clear increase in the AChE activity by 10(-4) M TTFD, a weak increase with 10(-5) M and an extremely weak increase, about the same as that in only the nutrient (Locke's solution), with 5 x 10(-4) M. However, with 10(-4) M of thiamine there was a tendency for the activity to be the same degree as with Lock's solution alone or be even inhibited at times.

摘要

通过组织学和超细胞化学研究，考察硫胺素及其二硫化物衍生物四氢糠基硫胺素二硫化物（TTFD）对摘除的豚鼠心房中乙酰胆碱酯酶（AChE）的影响，结果发现，10⁻⁴M的TTFD可使AChE活性明显增加，10⁻⁵M时增加较弱，而5×10⁻⁴M时增加极弱，与仅在营养液（洛克氏溶液）中的增加程度大致相同。然而，10⁻⁴M的硫胺素有时会使活性与仅在洛克氏溶液中的程度相同，甚至有时会受到抑制。