Nose Y, Lee J M, Ueno T, Hatakeyama M, Oishi K
Graduate School of Science and Technology, Kobe University, Japan.
Insect Biochem Mol Biol. 1997 Dec;27(12):1047-56. doi: 10.1016/s0965-1748(97)00091-x.
The cDNA for vitellogenin (Vg) of the parasitoid wasp Pimpla nipponica (Hymenoptera: Apocrita) was cloned and sequenced. The deduced amino acid sequence with 1807 residues was obtained. The N-terminal 20 amino acids chemically determined for vitellin (Vn) agreed completely with the deduced 20 amino acids that follow the 16 amino acid residues for putative signal peptide. The cDNA clone for the Vg of the turnip sawfly Athalia rosae (Hymenoptera: Symphyta), previously obtained and partially sequenced, was also completely sequenced and the amino acid sequence deduced. Amino acid sequences were compared between these two species and also with known Vg sequences from other insects. Common to all these insects is the presence of two long regions with relatively well-conserved amino acid sequences, one near the N-terminal extending 267-282 residues (including two cysteines at conserved locations), and the other starting at position 450 to 655 and extending 279-283 residues, and of a region at the C-terminal extending some 200 residues (about 250 in Aedes aegypti due to the presence of a serine-rich stretch) with 10 cysteines at conserved locations. A molecular phylogenetic tree was constructed.
克隆并测序了寄生蜂日本绒茧蜂（膜翅目：姬蜂科）卵黄原蛋白（Vg）的cDNA。获得了具有1807个残基的推导氨基酸序列。化学测定的卵黄磷蛋白（Vn）N端20个氨基酸与推测信号肽的16个氨基酸残基之后推导的20个氨基酸完全一致。之前已获得并部分测序的芜菁叶蜂（膜翅目：叶蜂科）Vg的cDNA克隆也进行了全序列测定并推导了氨基酸序列。比较了这两个物种之间以及与其他昆虫已知Vg序列的氨基酸序列。所有这些昆虫的共同之处在于存在两个氨基酸序列相对保守的长区域，一个在N端附近，延伸267 - 282个残基（包括保守位置的两个半胱氨酸），另一个从第450位到655位开始，延伸279 - 283个残基，以及在C端延伸约200个残基的区域（埃及伊蚊中由于存在富含丝氨酸的片段约为250个残基），在保守位置有10个半胱氨酸。构建了分子系统发育树。
