Use of rotary shadowing electron microscopy to investigate the collagen fibrils in the extracellular matrix of cuttle-fish (Sepia officinalis) and chicken cartilage.

Collagen fibrils isolated from sternal cartilage of chick embryo and chondrocranium of cuttle-fish (Sepia officinalis) were examined with the electron microscope after rotary showing. The aim was to determine whether collagen fibrils from S. officinalis cartilage contained collagen molecules similar to the type IX collagen of vertebrate cartilage. Cartilage from both sources presented a highly variable appearance and only occasionally did preparations contain fibrils having the structure described by Vaughan et al. (1988) for vertebrate cartilage. Subsequent electron microscope investigation of collagen samples during the various stages of fibril preparation showed that the method did not yield reproducible results, and that is altered the morphology of the isolated structures. It was not, therefore, possible to confirm the hypothesis that collagen molecules with a morphology similar to that type IX vertebrate collagen are a component of the extracellular matrix of cephalopod cartilage.