Aarts H J, van Lith L A, Keijer J
DLO-State Institute of Quality Control of Agricultural Products (RIKILT-DLO), Centre De Born, Wageningen, The Netherlands.
Lett Appl Microbiol. 1998 Feb;26(2):131-5. doi: 10.1046/j.1472-765x.1998.00302.x.
High resolution AFLP fingerprinting, in which subsets of genomic restriction fragments are amplified by means of PCR, was used for the identification of different. Salmonella serotypes to investigate whether this technique is applicable in epidemiological studies. Seventy-eight different Salmonella strains comprising 62 serotypes were genetically identified by AFLP. Primer combination M00 (MseI primer without additional 3' nucleotides) and E11 (EcoRI primer with two additional 3' nucleotides) resulted in reproducible profiles containing approximately 50 bands. All serotypes were characterized by a unique profile. In addition, AFLP fingerprinting enabled phage type identification. Different strains previously identified as identical, using typing methods with lower resolution, could be distinguished, showing that AFLP fingerprinting is well suited for bacterial epidemiology and identification.
高分辨率扩增片段长度多态性（AFLP）指纹图谱技术是通过PCR扩增基因组限制性片段的子集，用于鉴定不同的沙门氏菌血清型，以研究该技术是否适用于流行病学研究。通过AFLP对78株不同的沙门氏菌菌株（包括62个血清型）进行了基因鉴定。引物组合M00（无额外3'核苷酸的MseI引物）和E11（有两个额外3'核苷酸的EcoRI引物）产生了可重复的图谱，包含约50条带。所有血清型都具有独特的图谱特征。此外，AFLP指纹图谱技术还能够进行噬菌体分型鉴定。使用分辨率较低的分型方法先前鉴定为相同的不同菌株可以被区分开来，这表明AFLP指纹图谱技术非常适合细菌流行病学和鉴定。
