Glazier S A, Campbell E R, Campbell W H
Biotechnology Division, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, USA.
Anal Chem. 1998 Apr 15;70(8):1511-5. doi: 10.1021/ac971146s.
The construction and characterization of a nitrate reductase-based amperometric electrode for determination of nitrate ion is described. The electrode consisted of nitrate reductase held by dialysis membrane onto a Nafion-coated glassy carbon electrode. Methyl viologen was allowed to absorb into the Nafion layer, which acted as a reservoir for the electron mediator. The utility of the electrode to assay fertilizer and water sample for nitrate was demonstrated. The assays conducted with this electrode compared well with colorimetric and potentiometric assays of the same samples.
本文描述了一种基于硝酸还原酶的用于测定硝酸根离子的安培电极的构建与表征。该电极由通过透析膜固定在涂有Nafion的玻碳电极上的硝酸还原酶组成。使甲基紫精吸收到Nafion层中，该层作为电子媒介体的储存库。证明了该电极在测定肥料和水样中硝酸盐方面的实用性。用该电极进行的测定与相同样品的比色法和电位法测定结果相当。
