毛细管气相色谱法结合原子发射检测用于分析滴滴涕及其代谢物。

Capillary gas chromatography combined with atomic emission detection for the analysis of DDT and metabolites.

作者信息

Semb S I, Brevik E M, Pedersen-Bjergaard S

机构信息

Norwegian Institute for Water Research, Oslo, Norway.

出版信息

Chemosphere. 1998 Jan;36(2):213-24. doi: 10.1016/s0045-6535(97)00277-4.

DOI:10.1016/s0045-6535(97)00277-4

PMID:9569934

Abstract

Capillary gas chromatography with atomic emission detection (GC-AED) was evaluated for the determination of DDT and metabolites in biological samples. Utilizing chlorine-selective detection at 479 nm, DDT, DDE, and DDD were quantified down to the 20 ng/g (fat weight) level (S/N = 10) in cod liver oil, while the detection, limit was 6 ng/g (S/N = 3). With splitless injection performed in the pressure programmed mode, DDT and related compounds were quantified based on a single chlorine calibration curve (universal calibration). The quantitative data obtained by GC-AED were in excellent accordance with similar results from capillary gas chromatography with electron capture detection (GC-ECD), while the procedure for calibration was simplified with the former technique.

摘要

采用带原子发射检测的毛细管气相色谱法（GC-AED）对生物样品中的滴滴涕及其代谢物进行了测定评估。利用479 nm处的氯选择性检测，在鳕鱼肝油中，滴滴涕、滴滴伊和滴滴滴的定量下限为20 ng/g（脂肪重量）（信噪比=10），而检测限为6 ng/g（信噪比=3）。在程序升温模式下进行不分流进样，基于单一氯校准曲线（通用校准）对滴滴涕及相关化合物进行定量。GC-AED获得的定量数据与毛细管气相色谱-电子捕获检测法（GC-ECD）的类似结果高度一致，同时前一种技术简化了校准程序。