Ree G E, Payne J F
Department of Biology, Memorial University of Newfoundland, St. John's, Canada.
Chemosphere. 1997 Feb;34(4):855-67. doi: 10.1016/s0045-6535(97)00016-7.
The effect of Toxaphene on fish reproduction was investigated in sexually mature female zebrafish fed for two weeks with food contaminated with three different concentrations of the Pesticide (0.02, 0.23 and 2.2 micrograms/g fish/day). No overt differences were observed in reproductive success as assessed by examination of (a) total number of eggs spawned by each female, (b) percentage of fertilized eggs 24 hours after fertilization, (c) percentage of embryo mortality 72 hours after fertilization and (d) percentage of hatching 72 hours after fertilization. By contrast Toxaphene produced distinct effects of a dose response nature on oviposition. This observation is not only of interest with respect to Toxaphene, but also points to the importance of evaluating other pesticides for their effect on the oviposition of feral species that might be even more sensitive than zebrafish. Toxicity of Toxaphene was manifested both in the parent fish (skin discoloration, subcutaneous hemorrhages, particularly around the gill areas and backbones curved in the vertical plane) and in hatching embryos (half hatched).
在性成熟的雌性斑马鱼中研究了毒杀芬对鱼类繁殖的影响，这些斑马鱼被喂食了被三种不同浓度的该农药（0.02、0.23和2.2微克/克鱼/天）污染的食物，持续两周。通过检查以下指标评估繁殖成功率时，未观察到明显差异：（a）每条雌性产卵的总数；（b）受精后24小时受精卵的百分比；（c）受精后72小时胚胎死亡率的百分比；（d）受精后72小时孵化率的百分比。相比之下，毒杀芬对产卵产生了明显的剂量反应性质的影响。这一观察结果不仅对于毒杀芬而言很有趣，还指出了评估其他农药对野生物种产卵影响的重要性，这些野生物种可能比斑马鱼更为敏感。毒杀芬的毒性在亲鱼（皮肤变色、皮下出血，特别是在鳃部和垂直平面弯曲的脊椎周围）和孵化的胚胎（半孵化）中均有表现。
