Kiss R, Decaestecker C, Camby I, Darro F, Salmon I, Danguy A, Pasteels J L, Yeaton P
Laboratoire d'Histologie, Faculté de Médecine, Hôpital Erasme, Université Libre de Bruxelles, Brussels, Belgium.
Anal Quant Cytol Histol. 1998 Apr;20(2):133-43.
We investigated whether a relationship exists in terms of growth pattern and hormone sensitivity in 18 gastrointestinal neoplastic cell lines. Hormones studied included gastrin, epidermal growth factor, estradiol and luteinizing hormone-releasing hormone.
The growth patterns were assessed by means of computer-assisted microscope analysis of Feulgen-stained nuclei combined with the mathematical Delaunay triangulation and Voronoi paving techniques. This methodology enabled four variables characterizing the cell colony patterns to be computed. The information contributed by these variables was analyzed by means of discriminant analysis and the decision tree technique.
Each phenotype (sensitivity level) exhibited distinct growth pattern (or cell colony) characteristics in the case of each hormone and/or growth factor under study. Furthermore, the sensitivity of the gastrointestinal cell lines to a given hormone (or growth factor) appeared to be peculiar to the hormone (or growth factor).
A direct relationship seems to exist between growth pattern and hormone sensitivity levels in gastrointestinal cancers, particularly colorectal.
我们研究了18种胃肠道肿瘤细胞系在生长模式和激素敏感性方面是否存在关联。所研究的激素包括胃泌素、表皮生长因子、雌二醇和促黄体生成素释放激素。
通过对福尔根染色细胞核进行计算机辅助显微镜分析，并结合数学上的德劳内三角剖分和沃罗诺伊镶嵌技术来评估生长模式。这种方法能够计算出表征细胞集落模式的四个变量。通过判别分析和决策树技术对这些变量所提供的信息进行分析。
在所研究的每种激素和/或生长因子的情况下，每种表型（敏感性水平）都表现出独特的生长模式（或细胞集落）特征。此外，胃肠道细胞系对特定激素（或生长因子）的敏感性似乎因激素（或生长因子）而异。
胃肠道癌症，尤其是结直肠癌，其生长模式与激素敏感性水平之间似乎存在直接关联。
