Consiglio R, Rengo S, Liguoro D, Riccitiello F, Formisano S, Palumbo G, Di Jeso B
Istituto di Discipline Odontostomatologiche, Facoltà di Medicina e Chirurgia, Italy.
Arch Oral Biol. 1998 Jan;43(1):65-71. doi: 10.1016/s0003-9969(97)00087-3.
The effects of several glass-ionomer cements (Baseline and Chem Fil of De Trey; Ketac Fil and Ketac Bond of ESPE; Vitrebond and Vitremer of 3 M) on the protein synthesis of cultured gingival fibroblasts were studied. The presence of cements in the culture medium inhibited protein synthesis, although the relative effectiveness varied significantly. The cements tested have been ranked in three groups, group A (Chem Fil and Ketac Fil), group B (Ketac Bond and Baseline) and group C (Vitrebond and Vitremer), showing 50, 75 and 100% reduction in protein synthesis, respectively. Incubation of cells with medium previously conditioned by Baseline and Ketac Fil caused only transient inhibition of protein synthesis followed by almost complete recovery. This recovery was not observed when the medium was conditioned with Vitrebond. A characterization of the factors determining the inhibitory effect of one cement in each group was attempted. The cements, irrespective of the type, produced small but reproducible decreases in the pH of the medium but released fluoride ions to a different extent. Both changes in the pH of the medium and in the fluoride concentration inhibited protein synthesis by cultured gingival fibroblasts. The different action of cements can be explained, at least in part, by a differing release of fluoride ions.
研究了几种玻璃离子水门汀（德瑞的基线型和化学型；贺利氏的凯塔型和凯塔粘结型；3M的维他邦和维他美）对培养的牙龈成纤维细胞蛋白质合成的影响。尽管相对有效性差异显著，但培养基中存在水门汀会抑制蛋白质合成。所测试的水门汀被分为三组，A组（化学型和凯塔型）、B组（凯塔粘结型和基线型）和C组（维他邦和维他美），分别显示蛋白质合成减少50%、75%和100%。用先前经基线型和凯塔型处理过的培养基孵育细胞仅导致蛋白质合成的短暂抑制，随后几乎完全恢复。当用维他邦处理培养基时，未观察到这种恢复。尝试对每组中决定一种水门汀抑制作用的因素进行表征。无论哪种类型，水门汀都会使培养基的pH值产生微小但可重复的下降，但释放氟离子的程度不同。培养基pH值的变化和氟离子浓度的变化均抑制培养的牙龈成纤维细胞的蛋白质合成。水门汀的不同作用至少部分可以通过氟离子的不同释放来解释。
