The in vivo activity of vascular endothelial growth factor isoforms in the avian embryo.

The activity of the different isoforms of quail vascular endothelial growth factor (VEGF; 122, 146, 166 and 190 amino acids) were quantitatively examined in vivo by overexpression in the chicken embryo using the retroviral expression vector RCAS. All isoforms were potent inducers of vascularization and permeability. A linear relationship between expression of their mRNA and induction of vascularization and permeability was observed for all isoforms. Pattern formation and morphogenesis was otherwise not altered. Overexpression of qVEGF122 and 146 in the eye of chicken embryos did not induce vascularization of either the cornea or retina-which are avascular tissues in birds. We conclude that all isoforms of VEGF are potent inducers of angiogenesis dependent on a permissive environment.