DeMarino S, Olson L E, Pou N A, Adams S U, Harris T R
Johnson and Johnson Medical, Inc., Tampa, FL, USA.
Ann Biomed Eng. 1998 May-Jun;26(3):417-30. doi: 10.1114/1.94.
The objective of this study was a validation of an optical multiple indicator dilution technique for measuring microvascular exchange parameters in edematous lungs by comparison to conventional radioisotope multiple indicator dilution methods. Six anesthetized dogs were studied at baseline and after alloxan infusion to increase capillary permeability. In addition, 11 isolated, perfused dog lungs were studied at baseline and after edema was created by increasing venous pressure or by infusing alloxan to increase vascular permeability. Increased capillary permeability from alloxan infusion led to increases in most but not all capillary exchange parameters as analyzed by mathematical models and measured by both optical and radioisotope methods. Increased vascular pressure led to increased edema but no significant increases in capillary exchange parameters. Two-way analysis of variance (ANOVA; variations in baseline versus pressure or alloxan and variation in optical versus radioisotope for each transport parameter derived from the mathematical models) indicated few significant differences in capillary exchange parameters between optical and radioisotope measures. Newman-Keuls multiple comparison tests did uncover some variations between a few of the group-mean values derived from optical and radioisotope methods. However, optical and radioisotope parameter measurements were highly correlated for all studies regardless of the mathematical model used for analysis.
本研究的目的是通过与传统放射性同位素多指示剂稀释法相比较，验证一种用于测量水肿肺微血管交换参数的光学多指示剂稀释技术。对6只麻醉犬在基线状态以及注射四氧嘧啶以增加毛细血管通透性后进行了研究。此外，对11个离体灌注的犬肺在基线状态以及通过增加静脉压或注射四氧嘧啶以增加血管通透性从而造成水肿后进行了研究。通过数学模型分析并用光学和放射性同位素方法测量发现，注射四氧嘧啶导致的毛细血管通透性增加使大多数（但并非所有）毛细血管交换参数升高。血管压力增加导致水肿加剧，但毛细血管交换参数无显著升高。双向方差分析（ANOVA；数学模型得出的各转运参数在基线与压力或四氧嘧啶之间的变化以及光学与放射性同位素之间的变化）表明，光学测量与放射性同位素测量所得的毛细血管交换参数之间几乎没有显著差异。纽曼-基尔斯多重比较检验确实发现，从光学和放射性同位素方法得出的一些组均值之间存在一些差异。然而，无论用于分析的数学模型如何，在所有研究中光学和放射性同位素参数测量均高度相关。
