Qin G, Su J, Ning Y, Duan X, Luo D, Lotlikar P D
Fels Institute for Cancer Research and Molecular Biology and Department of Biochemistry, Temple University School of Medicine, Philadelphia, PA 19140, USA.
Cancer Lett. 1997 Dec 23;121(2):203-10. doi: 10.1016/s0304-3835(97)00352-2.
Mutation of the p53 gene has been reported in hepatocellular carcinoma (HCC) occurring worldwide. The most frequent p53 mutation has been found in HCCs in regions with high hepatitis B virus (HBV) infection and intake of aflatoxin B1 (AFB1). The aim of our study was to examine p53 protein expression in HCCs from a high incidence area of Guangxi, Southern China, where HBV infection and dietary intake of AFB1 are high. Immunohistochemical staining of p53 protein was carried out using a polyclonal rabbit antibody (CM-1). Serial sections were also stained for hepatitis B surface antigen and core antigen. p53 Protein expression was detected in 13 (43.3%) of the 30 HCCs. Expression of p53 was found in 25.0% (1/4) of the < or = 5.0 cm diameter HCCs, in 36.8% (7/19) of the 5.1-10.0 cm diameter HCCs and in 71.4% (5/7) of the >10.0 cm diameter HCCs. Expression of p53 was observed more in moderately and poorly differentiated than in the well differentiated HCCs and more frequently seen in HCCs from younger patients. These data indicate that there is a close association between p53 protein expression and tumor size, histological grade and age of patients. Twenty-seven out of 30 cases (90.0%) were positive for HBV. No significant association between p53 expression and sex. HBV infection, cirrhosis or alpha-fetoprotein has been found.
全球范围内的肝细胞癌（HCC）中均有p53基因突变的报道。在乙型肝炎病毒（HBV）感染率高且黄曲霉毒素B1（AFB1）摄入量高的地区，HCC中最常见的p53突变已被发现。我们研究的目的是检测中国南方广西肝癌高发地区HCC中p53蛋白的表达情况，该地区HBV感染率和AFB1饮食摄入量均较高。使用兔多克隆抗体（CM - 1）对p53蛋白进行免疫组织化学染色。连续切片还进行了乙型肝炎表面抗原和核心抗原染色。在30例HCC中，有13例（43.3%）检测到p53蛋白表达。直径≤5.0 cm的HCC中，p53表达率为25.0%（1/4）；直径5.1 - 10.0 cm的HCC中，表达率为36.8%（7/19）；直径＞10.0 cm的HCC中，表达率为71.4%（5/7）。与高分化HCC相比，中低分化HCC中p53表达更多，且在年轻患者的HCC中更常见。这些数据表明p53蛋白表达与肿瘤大小、组织学分级和患者年龄密切相关。30例中有27例（90.0%）HBV阳性。未发现p53表达与性别、HBV感染、肝硬化或甲胎蛋白之间存在显著关联。
