Di Cola A, Poma A, Spanò L
Dipartimento di Biologia di Base ed Applicata, Università degli Studi de L'Aquila, Italy.
Cell Biol Int. 1997 Sep;21(9):595-600. doi: 10.1006/cbir.1997.0190.
The pattern of expression of the rolB gene, derived from the T-DNA of the plant pathogen Agrobacterium rhizogenes, has been investigated during the early stages of somatic embryo formation in suspension cultures of carrot (Daucus carota L.). The reporter gene GUS (Escherichia coli beta-glucuronidase), under transcriptional control of full-length rolB promoter region, has been utilized in order to evaluate both qualitative and quantitative variations in the expression pattern. Fluorimetric measurements point to the developmental regulation of the gene, while results from histochemical analysis indicate that the promoter of rolB is firstly activated in the central (core) region of the globular embryos.
在胡萝卜（Daucus carota L.）悬浮培养物体细胞胚胎形成的早期阶段，对源自植物病原菌发根农杆菌T-DNA的rolB基因的表达模式进行了研究。利用在全长rolB启动子区域转录控制下的报告基因GUS（大肠杆菌β-葡萄糖醛酸酶）来评估表达模式的定性和定量变化。荧光测量表明该基因受发育调控，而组织化学分析结果表明rolB启动子首先在球形胚的中央（核心）区域被激活。
