Steiner F, Weber K, Fürst D O
Department of Biochemistry, Max-Planck-Institute for Biophysical Chemistry, Göttingen, Germany.
Genomics. 1998 Apr 1;49(1):83-95. doi: 10.1006/geno.1998.5220.
The complete exon-intron organization of the murine gene encoding M-protein, a structural protein of sarcomeric myofibrils, was determined. The gene is composed of 37 exons and 36 introns, spanning approximately 75 kb of DNA. Intron positions are related to the modular structure of M-protein, which is composed essentially of immunoglobulin and fibronectin type III domains. Almost all repeats follow a two exon-one domain structure. The beginning and end of each domain are defined by introns in phase I; internal introns are more divergent in position and very rarely use phase I. A single transcriptional start point was detected in both skeletal and cardiac muscle. Analysis of the prospective promoter region revealed several potential regulatory elements. CAT expression assays using promoter deletion constructs identified three regions that seem to be most important for the muscle-specific transcription activation of the M-protein gene. These results provide the first complete characterization of a gene for a member of the intracellular branch of the immunoglobulin superfamily.
确定了编码肌节肌原纤维结构蛋白M蛋白的小鼠基因的完整外显子-内含子结构。该基因由37个外显子和36个内含子组成，跨越约75 kb的DNA。内含子位置与M蛋白的模块化结构相关，M蛋白主要由免疫球蛋白和III型纤连蛋白结构域组成。几乎所有的重复序列都遵循两个外显子-一个结构域的结构。每个结构域的起始和末端由I期内含子界定；内部内含子在位置上差异更大，很少使用I期。在骨骼肌和心肌中均检测到单个转录起始点。对预期启动子区域的分析揭示了几个潜在的调控元件。使用启动子缺失构建体的CAT表达分析确定了三个似乎对M蛋白基因的肌肉特异性转录激活最为重要的区域。这些结果首次完整地描述了免疫球蛋白超家族细胞内分支成员的一个基因。
