Kinjo J, Hatakeyama M, Udayama M, Tsutanaga Y, Yamashita M, Nohara T, Yoshiki Y, Okubo K
Faculty of Pharmaceutical Sciences, Kumamoto University, Japan.
Biosci Biotechnol Biochem. 1998 Mar;62(3):429-33. doi: 10.1271/bbb.62.429.
HPLC analysis and yield of oleanene-glucuronide (OG) was done on some commercially available edible beans: seeds of Glycine max, Glycine max cv. Kuromame, Phaseolus vulgaris cv. Torosuku, Phaseolus vulgaris cv. Toramame, Phaseolus vulgaris cv. Taishokintoki, Phaseolus coccineus cv. Ooshirobana, Phaseolus coccineus cv. Murasakihanamame, Vigna unguiculata cv. Chuguro, Vigna angularis cv. Dainagon, Arachis hypogaea, Pisum sativum, and Vicia faba. All the beans listed above have OG, though in varying the amounts. Furthermore the HPLC profiles of beans belonging to the same genus were very similar except for that of the Vigna genus. There was no great difference of the HPLC profiles with respect to the cultivated varieties. The structures of the major OGs in each type of beans were identified as soyasaponins I (1) and V (2), and phaseoside I (3) (Phaseolus vulgaris and P. coccineus); 1 and soyasaponin II (4) (Glycine max); 1 and 2 (Vigna unguiculata); 1 (Pisum sativum, Arachis hypogaea and Vicia faba). In contrast, those in Dainagon (Vigna angularis cv. Dainagon) were identified as azukisaponins II (5) and VI (6).
对一些市售食用豆类进行了齐墩果烯葡糖醛酸苷（OG）的高效液相色谱（HPLC）分析及产量测定，这些豆类包括：大豆种子、大豆品种黒豆、菜豆品种托罗素库、菜豆品种托拉豆、菜豆品种大正金时、多花菜豆品种大白雪豆、多花菜豆品种紫花菜豆、豇豆品种中黒、赤小豆品种大纳言、落花生、豌豆和蚕豆。上述所有豆类均含有OG，不过含量各不相同。此外，除豇豆属外，同一属豆类的HPLC图谱非常相似。不同栽培品种的HPLC图谱差异不大。各类豆类中主要OG的结构鉴定如下：大豆皂苷I（1）和V（2）以及菜豆苷I（3）（菜豆和多花菜豆）；1和大豆皂苷II（4）（大豆）；1和2（豇豆）；1（豌豆、落花生和蚕豆）。相比之下，大纳言（赤小豆品种大纳言）中的OG鉴定为小豆皂苷II（5）和VI（6）。
