Aslam I, Fishel S
CARE, The Park Hospital, Arnold, Nottingham, UK.
Hum Reprod. 1998 Mar;13(3):634-8. doi: 10.1093/humrep/13.3.634.
Testicular cell suspensions were prepared from obstructive and non-obstructive azoospermic men and were cultured in vitro for 96 h as (i) mixed cell populations and (ii) isolated homogeneous populations of primary spermatocytes, round spermatids and elongating spermatids. The cells lost their viability gradually during the first 24 h period. By 72 h almost 90% of the cells were non-viable. Isolated pure fractions showed better viability at each time interval (P < 0.0005). Throughout the culture period primary spermatocytes, elongating spermatids and other non-spermatogenic cells showed no change in their morphology, but almost 22% of round spermatids showed growth of flagella. Most of the round spermatids developed their flagella during the first 4-8 h period of culture. Isolated pure round spermatids showed better flagellar growth compared with mixed cell suspensions (P < 0.0005). The spermatogenic cells were successfully cryopreserved. However, when mixed spermatogenic cell suspensions were cryopreserved, more cells lost their viability compared with when isolated pure fractions were cryopreserved (P < 0.0005).
从梗阻性和非梗阻性无精子症男性中制备睾丸细胞悬液，并将其作为（i）混合细胞群体和（ii）分离的原代精母细胞、圆形精子细胞和伸长精子细胞的同质群体进行体外培养96小时。细胞在最初的24小时内逐渐失去活力。到72小时时，几乎90%的细胞已无活力。分离的纯组分在每个时间间隔显示出更好的活力（P < 0.0005）。在整个培养期间，原代精母细胞、伸长精子细胞和其他非生精细胞的形态没有变化，但几乎22%的圆形精子细胞出现鞭毛生长。大多数圆形精子细胞在培养的最初4 - 8小时内长出鞭毛。与混合细胞悬液相比，分离的纯圆形精子细胞显示出更好的鞭毛生长（P < 0.0005）。生精细胞成功冷冻保存。然而，与分离的纯组分冷冻保存相比，混合生精细胞悬液冷冻保存时更多细胞失去活力（P < 0.0005）。
