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环磷酸腺苷（cAMP）-cAMP受体蛋白复合物对IS2转座的负调控

Negative regulation of IS2 transposition by the cyclic AMP (cAMP)-cAMP receptor protein complex.

作者信息

Hu S T, Wang H C, Lei G S, Wang S H

机构信息

Department of Microbiology and Graduate Institute of Microbiology and Immunology, National Yang-Ming University, Taipei, Taiwan, Republic of China.

出版信息

J Bacteriol. 1998 May;180(10):2682-8. doi: 10.1128/JB.180.10.2682-2688.1998.

DOI:10.1128/JB.180.10.2682-2688.1998
PMID:9573153
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC107220/
Abstract

Three sequences similar to that of the consensus binding sequence of the cyclic AMP (cAMP)-cAMP receptor protein (CRP) complex were found in the major IS2 promoter region. Experiments were performed to determine whether the cAMP-CRP complex plays a role in the regulation of IS2 transposition. In the gel retardation assay, the cAMP-CRP complex was found to be able to bind the major IS2 promoter. A DNA footprinting assay confirmed that the cAMP-CRP complex binds to the sequences mentioned above. With an IS2 promoter-luciferase gene fusion construct, the cAMP-CRP complex was shown to inhibit transcription from the major IS2 promoter. IS2 was found to transpose at a frequency approximately 200-fold higher in an Escherichia coli host defective for CRP or adenyl cyclase than in a wild-type host. These results suggest that the cAMP-CRP complex is a negative regulator of IS2 transposition.

摘要

在主要的IS2启动子区域发现了三个与环磷酸腺苷（cAMP）-cAMP受体蛋白（CRP）复合物的共有结合序列相似的序列。进行了实验以确定cAMP-CRP复合物是否在IS2转座调控中发挥作用。在凝胶阻滞试验中，发现cAMP-CRP复合物能够结合主要的IS2启动子。DNA足迹试验证实cAMP-CRP复合物与上述序列结合。利用IS2启动子-荧光素酶基因融合构建体，显示cAMP-CRP复合物抑制主要IS2启动子的转录。发现在缺乏CRP或腺苷酸环化酶的大肠杆菌宿主中，IS2的转座频率比野生型宿主高约200倍。这些结果表明，cAMP-CRP复合物是IS2转座的负调节因子。

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