大鼠精子顶体穿孔器的分离与鉴定
Isolation and characterization of the perforatorium of rat spermatozoa.
作者信息
Olson G E, Hamilton D W, Fawcett D W
出版信息
J Reprod Fertil. 1976 Jul;47(2):293-7. doi: 10.1530/jrf.0.0470293.
PMID:957329
Abstract
The perforatorium of rat spermatozoa was isolated and its protein composition determined. SDS-polyacrylamide gel electrophoresis showed that the organelle is composed of a single polypeptide component with a molecular weight of 13,000. The perforatorium becomes more resistant to solubilization during epididymal transit due to an apparent increase in disulphide bond content. Amino acid analysis of the perforatorium polypeptide revealed a content of 6-5% cysteine.
摘要
分离出大鼠精子的穿孔器并测定其蛋白质组成。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳显示，该细胞器由单一的多肽成分组成，分子量为13000。由于二硫键含量明显增加，穿孔器在附睾转运过程中对溶解的抵抗力增强。对穿孔器多肽的氨基酸分析显示半胱氨酸含量为6 - 5%。
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