Hess G F, Drong R F, Weiland K L, Slightom J L, Sclafani R A, Hollingsworth R E
Cancer Research, Pharmacia, Upjohn, Inc., 301 Henrietta Street, Kalamazoo, MI 49001, USA.
Gene. 1998 Apr 28;211(1):133-40. doi: 10.1016/s0378-1119(98)00094-8.
The Cdc7 protein kinase of Saccharomyces cerevisiae is a critical regulator of several aspects of DNA metabolism and cell cycle progression. We describe the isolation of a human gene encoding a Cdc7 homolog. The Cdc7Hs protein sequence is 27% identical to that of the yeast protein, includes features unique to yeast Cdc7, and contains all conserved catalytic residues of protein kinases. The human sequence also shows significant similarity to the cyclin-dependent kinases, in accordance with evidence that yeast Cdc7 is related to the cdks. CDC7Hs is expressed in many normal tissues, but overexpressed in certain tumor types and all transformed cell lines examined. In some of the tumors tested, CDC7Hs expression correlates with expression of a proliferation marker, the histone H3 gene. In other cases, no such correlation was observed. This suggests that CDC7Hs expression may be associated hyperproliferation in some tumors and neoplastic transformation in others.
酿酒酵母的Cdc7蛋白激酶是DNA代谢和细胞周期进程多个方面的关键调节因子。我们描述了一个编码Cdc7同源物的人类基因的分离。Cdc7Hs蛋白序列与酵母蛋白的序列有27%的同一性，包含酵母Cdc7特有的特征，并含有蛋白激酶所有保守的催化残基。根据酵母Cdc7与细胞周期蛋白依赖性激酶相关的证据，人类序列与细胞周期蛋白依赖性激酶也显示出显著的相似性。CDC7Hs在许多正常组织中表达，但在某些肿瘤类型和所有检测的转化细胞系中过表达。在一些测试的肿瘤中，CDC7Hs的表达与增殖标记物组蛋白H3基因的表达相关。在其他情况下，未观察到这种相关性。这表明CDC7Hs的表达在某些肿瘤中可能与过度增殖相关，而在其他肿瘤中可能与肿瘤转化相关。
