Collins M D, Hutson R A, Falsen E, Sjöden B, Facklam R R
Department of Microbiology, BBSRC Institute of Food Research, Reading Laboratory, United Kingdom.
J Clin Microbiol. 1998 May;36(5):1290-3. doi: 10.1128/JCM.36.5.1290-1293.1998.
Six strains of a hitherto-undescribed gram-positive, catalase-negative, facultatively anaerobic coccus from human sources were characterized by phenotypic and molecular taxonomic methods. Comparative 16S rRNA gene sequencing studies demonstrated that the unknown strains are genealogically homogeneous and constitute a new subline within the genus Gemella. The unknown bacterium was readily distinguished from Gemella haemolysans, the type species of the genus Gemella, and from Gemella morbillorum by biochemical tests and electrophoretic analysis of whole-cell proteins. On the basis of phylogenetic and phenotypic evidence, it is proposed that the unknown bacterium from clinical specimens be classified as Gemella bergeriae sp. nov. The type strain of G. bergeriae is CCUG 37817 (= strain 617-93).
采用表型和分子分类学方法对从人类样本中分离出的6株迄今未描述的革兰氏阳性、过氧化氢酶阴性、兼性厌氧球菌进行了鉴定。比较16S rRNA基因测序研究表明,这些未知菌株在谱系上是同源的,构成了Gemella属内的一个新亚系。通过生化试验和全细胞蛋白的电泳分析,该未知细菌很容易与Gemella属的模式种溶血孪生球菌以及麻疹孪生球菌区分开来。基于系统发育和表型证据,建议将临床样本中的未知细菌分类为新种伯杰孪生球菌(Gemella bergeriae sp. nov.)。伯杰孪生球菌的模式菌株为CCUG 37817(=菌株617-93)。