神经降压素在体外刺激大鼠肾上腺髓质释放促肾上腺皮质激素释放激素（CRH）和促肾上腺皮质激素（ACTH）。

Neurotensin stimulates CRH and ACTH release by rat adrenal medulla in vitro.

作者信息

Mazzocchi G, Malendowicz L K, Rebuffat P, Gottardo G, Nussdorfer G G

机构信息

Department of Anatomy, University of Padua, Padova, Italy.

出版信息

Neuropeptides. 1997 Feb;31(1):8-11. doi: 10.1016/s0143-4179(97)90011-1.

DOI:10.1016/s0143-4179(97)90011-1

PMID:9574829

Abstract

Neurotensin (NT) is a 13-amino acid peptide, widely distributed in the central and peripheral nervous system, which is able to stimulate the activity of the hypothalamo-pituitary CRH-ACTH system. We investigated by RIA the effect of NT on the release of CRH and ACTH immunoreactivities (ir) by rat adrenal medulla in vitro. NT enhanced the release of both CRH-ir and ACTH-ir, the maximal effective concentration being 10(-8) M. [D-Trp11]-NT, a specific NT receptor antagonist, abrogated the effects of NT. The stimulatory effect of 10(-8) M NT on ACTH-ir release was blocked by alpha-helical-CRH (an antagonist of CRH receptors), thereby suggesting that the enhancement in ACTH secretion is consequent to the stimulation of CRH release. These findings suggest that NT is a stimulator not only of the central (hypothalamo-pituitary), but also of the peripheral (intramedullary), branch of the CRH-ACTH system.

摘要

神经降压素（NT）是一种由13个氨基酸组成的肽，广泛分布于中枢和外周神经系统，能够刺激下丘脑 - 垂体CRH - ACTH系统的活性。我们通过放射免疫分析法（RIA）研究了NT对大鼠肾上腺髓质体外释放CRH和ACTH免疫反应性（ir）的影响。NT增强了CRH - ir和ACTH - ir的释放，最大有效浓度为10^(-8) M。[D - Trp11] - NT，一种特异性的NT受体拮抗剂，消除了NT的作用。10^(-8) M NT对ACTH - ir释放的刺激作用被α - 螺旋 - CRH（CRH受体拮抗剂）阻断，从而表明ACTH分泌的增强是由于CRH释放的刺激所致。这些发现表明，NT不仅是CRH - ACTH系统中枢（下丘脑 - 垂体）分支的刺激物，也是外周（髓质内）分支的刺激物。