Summers L K, Arner P, Ilic V, Clark M L, Humphreys S M, Frayn K N
Nuffield Department of Clinical Medicine, Radcliffe Infirmary, Oxford, United Kingdom.
Am J Physiol. 1998 Apr;274(4):E651-5. doi: 10.1152/ajpendo.1998.274.4.E651.
We investigated whether two different methods of studying metabolism in adipose tissue, microdialysis and the arteriovenous technique, produced comparable results during the postprandial period. Interstitial glycerol concentrations measured by microdialysis are usually used as an index of intracellular lipolysis, and it is not known whether they also reflect the intravascular action of lipoprotein lipase in the postprandial period. The two techniques were compared in 10 healthy subjects fed mixed meals. Interstitial glycerol concentrations reflected those measured in adipose tissue venous plasma. However, the calculation of the rate of glycerol release from adipose tissue using the microdialysis data differed systematically from that using arteriovenous difference measurement. The former method gave, on average, 40% lower values than the latter one. The difference is probably due to the assumptions that had to be made for the calculation of glycerol release. The two techniques have complementary places in the study of postprandial adipose tissue metabolism, with microdialysis reflecting intracellular hormone-sensitive lipase action rather than intravascular lipoprotein lipase.
我们研究了在餐后期间，两种不同的研究脂肪组织代谢的方法，即微透析法和动静脉技术，是否能产生可比的结果。通过微透析测量的间质甘油浓度通常被用作细胞内脂肪分解的指标，尚不清楚它们在餐后期间是否也反映脂蛋白脂肪酶的血管内作用。在10名食用混合餐的健康受试者中对这两种技术进行了比较。间质甘油浓度反映了在脂肪组织静脉血浆中测得的浓度。然而，使用微透析数据计算脂肪组织甘油释放率与使用动静脉差值测量法的计算结果存在系统性差异。前一种方法得出的值平均比后一种方法低40%。这种差异可能是由于计算甘油释放时所做的假设。这两种技术在餐后脂肪组织代谢研究中具有互补作用，微透析反映的是细胞内激素敏感性脂肪酶的作用，而非血管内脂蛋白脂肪酶的作用。
