Chauvet A E, Kesava P P, Goh C S, Badie B
Department of Medical Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, USA.
J Neurosurg. 1998 May;88(5):870-3. doi: 10.3171/jns.1998.88.5.0870.
The goal of this study was to evaluate gene delivery to a benign brain tumor.
A recombinant adenovirus vector bearing the Escherichia coli beta-galactosidase reporter gene was selectively injected into the vascular supply of a spontaneously occurring canine olfactory groove meningioma. The tumor and a small amount of peritumoral brain tissue were removed 5 days after viral injection and stained with X-Gal to assess gene delivery. The authors noted significant beta-galactosidase gene expression by the tumor, but not by surrounding brain tissue. No obvious viral-related cytotoxicity was noted.
The authors found that meningiomas can be successfully transduced by adenovirus vectors by using endovascular techniques.
本研究的目的是评估向良性脑肿瘤进行基因传递的情况。
将携带大肠杆菌β-半乳糖苷酶报告基因的重组腺病毒载体选择性地注入自发形成的犬嗅沟脑膜瘤的血管供应中。病毒注射5天后，切除肿瘤和少量肿瘤周围脑组织，用X-Gal染色以评估基因传递情况。作者注意到肿瘤中有显著的β-半乳糖苷酶基因表达，而周围脑组织中没有。未观察到明显的病毒相关细胞毒性。
作者发现，通过血管内技术，腺病毒载体可成功转导脑膜瘤。
