Wu G, Wieczorowska-Tobis K, Polubinska A, Korybalska K, Filas V, Tam P, French I, Breborowicz A
University of Toronto, Canada.
Perit Dial Int. 1998 Mar-Apr;18(2):217-24.
To evaluate the effect of supplementation of dialysis fluid with N-acetylglucosamine (NAG) on the permeability of peritoneum during chronic peritoneal dialysis in rats.
Experiments were performed on rats with surgically implanted peritoneal catheters. Dialysis solution [Dianeal 1.5% (Baxter, Deerfield, IL, U.S.A.) supplemented with either NAG 50 mmol/L or glucose 50 mmol/L (control)] was infused intraperitoneally twice, every day, for 8 weeks. Peritoneal equilibration tests (PET) were performed in all animals at the beginning of the study and after 8 weeks of dialysis. Additionally, at the end of each week, dialysis solution infused in the morning was drained after 4 hours of intraperitoneal dwell. White blood cell count, creatinine, and total protein concentrations were measured in the effluent dialysate. After 8 weeks of dialysis, the morphology of the peritoneum was studied.
In rats exposed to dialysis fluid supplemented with NAG, peritoneal permeability to creatinine and proteins was reduced when compared to animals dialyzed with glucose solution. In NAG treated animals, staining with alcian blue for polyanions in the peritoneal interstitium was significantly stronger than in rats dialyzed with glucose solution.
Chronic peritoneal dialysis with dialysis solution supplemented with N-acetylglucosamine causes accumulation of glycosaminoglycans in the peritoneal interstitium, which results in a change of peritoneal permeability.
评估在大鼠慢性腹膜透析过程中，向透析液中添加N - 乙酰葡糖胺（NAG）对腹膜通透性的影响。
对通过手术植入腹膜导管的大鼠进行实验。腹膜内每天两次注入透析液[1.5%的百特透析液（美国伊利诺伊州迪尔菲尔德市百特公司生产），添加50 mmol/L的NAG或50 mmol/L的葡萄糖（对照组）]，持续8周。在研究开始时和透析8周后，对所有动物进行腹膜平衡试验（PET）。此外，在每周结束时，将早晨注入的透析液在腹腔留置4小时后引流。测定流出的透析液中的白细胞计数、肌酐和总蛋白浓度。透析8周后，研究腹膜的形态。
与用葡萄糖溶液透析的动物相比，暴露于添加NAG的透析液的大鼠，其腹膜对肌酐和蛋白质的通透性降低。在接受NAG治疗的动物中，腹膜间质中用阿尔辛蓝染色的聚阴离子明显比用葡萄糖溶液透析的大鼠更强。
用添加N - 乙酰葡糖胺的透析液进行慢性腹膜透析会导致腹膜间质中糖胺聚糖的积累，从而导致腹膜通透性的改变。
