Belhacène N, Maulon L, Guérin S, Ricci J E, Mari B, Colin Y, Cartron J P, Auberger P
CJF INSERM 96. 05, Activation des Cellules Hématopoietiques, Faculté de Médecine, Nice, France.
FASEB J. 1998 May;12(7):531-9. doi: 10.1096/fasebj.12.7.531.
The erythroleukemic cell line K562 can undergo further differentiation in erythroid or megakaryocytic lineage depending on the nature of the stimulus. Phorbol ester (PMA) stimulates megakaryocytic development whereas hemin promotes erythroid differentiation of these cells. We have examined the effect of PMA and hemin on the expression of the Kell blood group and CD10 antigens, two related proteins that belong to a family of membrane-bound neutral metalloendopeptidases. We show here that differentiation of K562 cells by PMA in the megakaryocytic lineage results in abolishment of Kell mRNA accumulation and protein expression and, in parallel, the induction of CD10 mRNA accumulation, protein expression, and enzymatic activity. Conversely, differentiation of these cells by hemin in the erythroid lineage is accompanied by an up-regulation of Kell mRNA and protein expression, with no changes in CD10 mRNA and protein expression. Thus, CD10 and Kell can be regarded as specific markers of the differentiation of K562 cells in the megakaryocytic and erythroid lineages, respectively.
红白血病细胞系K562可根据刺激的性质在红系或巨核系中进一步分化。佛波酯（PMA）刺激巨核细胞发育，而血红素促进这些细胞的红系分化。我们研究了PMA和血红素对凯尔血型抗原和CD10抗原表达的影响，这两种相关蛋白属于膜结合中性金属内肽酶家族。我们在此表明，PMA诱导K562细胞在巨核系中分化会导致凯尔mRNA积累和蛋白表达的消失，同时，诱导CD10 mRNA积累、蛋白表达和酶活性。相反，血红素诱导这些细胞在红系中分化伴随着凯尔mRNA和蛋白表达的上调，而CD10 mRNA和蛋白表达没有变化。因此，CD10和凯尔可分别视为K562细胞在巨核系和红系中分化的特异性标志物。
