Tsuzuki T, Izumoto S, Ohnishi T, Hiraga S, Arita N, Hayakawa T
Department of Neurosurgery, Osaka University Medical School, Japan.
J Clin Pathol. 1998 Jan;51(1):13-7. doi: 10.1136/jcp.51.1.13.
To assess immunohistochemically whether the neural cell adhesion molecule L1, which is a member of the immunoglobulin superfamily and has been shown recently to be a stimulating factor for glioma migration, is expressed in glioma tissues, and to investigate factors that can regulate this expression.
Twenty seven glioma tissue specimens including 13 glioblastomas, seven anaplastic astrocytomas, and seven astrocytomas were examined. Immunohistochemical analyses of L1, p53, and transforming growth cell factor beta (TGF-beta) were performed on each tumour using both polyclonal and monoclonal antibodies.
Nine (33%) specimens (six glioblastomas and three anaplastic astrocytomas) had L1 positive immunostaining. p53 positive staining was detected in 10 (43%) of 23 glioma specimens (seven glioblastomas and three anaplastic astrocytomas). TGF-beta positive immunostaining was observed in 12 (52%) of the 23 glioma specimens (six glioblastomas, four anaplastic astrocytomas, and two astrocytomas). There was a statistical correlation between both p53 and L1 expression and TGF-beta and L1 expression. No such correlation was found between p53 and TGF-beta expression.
These results suggest that mutation of the p53 gene or expression of TGF-beta may upregulate the expression of the L1 gene, thus resulting in high grade migration of glioma cells.
通过免疫组织化学方法评估神经细胞黏附分子L1（免疫球蛋白超家族成员，最近被证明是胶质瘤迁移的刺激因子）是否在胶质瘤组织中表达，并研究可调节这种表达的因素。
检查了27个胶质瘤组织标本，包括13个胶质母细胞瘤、7个间变性星形细胞瘤和7个星形细胞瘤。使用多克隆和单克隆抗体对每个肿瘤进行L1、p53和转化生长细胞因子β（TGF-β）的免疫组织化学分析。
9个（33%）标本（6个胶质母细胞瘤和3个间变性星形细胞瘤）L1免疫染色呈阳性。在23个胶质瘤标本中的10个（43%）（7个胶质母细胞瘤和3个间变性星形细胞瘤）检测到p53阳性染色。在23个胶质瘤标本中的12个（52%）（6个胶质母细胞瘤、4个间变性星形细胞瘤和2个星形细胞瘤）观察到TGF-β阳性免疫染色。p53与L1表达以及TGF-β与L1表达之间存在统计学相关性。在p53与TGF-β表达之间未发现此类相关性。
这些结果表明，p53基因的突变或TGF-β的表达可能上调L1基因的表达，从而导致胶质瘤细胞的高度迁移。
