两性霉素B对人中性粒细胞体外白三烯B4合成的抑制作用。

Inhibitory effect of amphotericin B on leukotriene B4 synthesis in human neutrophils in vitro.

作者信息

Shindo K, Fukumura M, Ito A

机构信息

The First Department of Internal Medicine, Yokohama City University School of Medicine, Yokohama, Japan.

出版信息

Prostaglandins Leukot Essent Fatty Acids. 1998 Feb;58(2):105-9. doi: 10.1016/s0952-3278(98)90148-x.

DOI:10.1016/s0952-3278(98)90148-x

PMID:9578147

Abstract

Our objective was to determine the effect of amphotericin B on leukotriene (LT) A4 hydrolase in human neutrophil cytosol and to examine its effect on intact neutrophils in vitro. Cytosolic fractions were assayed for LTA4 hydrolase and 5-lipoxygenase activity in the presence or absence of amphotericin B. The IC50 of amphotericin B for LTA4 hydrolase activity was 0.72 microM. No inhibition of 5-lipoxygenase activity in the cytosolic fraction was detected. The IC50 of amphotericin B for leukotriene B4 synthesis in intact neutrophils was 0.43 microM. The 5-hydro(per) oxy-eicosatetraenoic acid (5-H(P)ETE) synthesis was diminished in intact cells by 66.8 [3.4]% (mean[SEM]) in the presence of 0.01 mM amphotericin B. Thus, amphotericin B inhibited the synthesis of LTB4 and 5-H(P)ETE in neutrophils in vitro. Differences between the results of studies on cytosol and on intact cells suggest that amphotericin B is involved in a complex interaction in the intact cell.

摘要

我们的目的是确定两性霉素B对人中性粒细胞胞质溶胶中白三烯（LT）A4水解酶的作用，并在体外检测其对完整中性粒细胞的影响。在有或没有两性霉素B的情况下，对胞质溶胶部分进行LTA4水解酶和5-脂氧合酶活性检测。两性霉素B对LTA4水解酶活性的IC50为0.72微摩尔。未检测到对胞质溶胶部分中5-脂氧合酶活性的抑制作用。两性霉素B对完整中性粒细胞中白三烯B4合成的IC50为0.43微摩尔。在存在0.01毫摩尔两性霉素B的情况下，完整细胞中5-氢（过）氧二十碳四烯酸（5-H(P)ETE）的合成减少了66.8 [3.4]%（平均值[标准误]）。因此，两性霉素B在体外抑制中性粒细胞中LTB4和5-H(P)ETE的合成。对胞质溶胶和完整细胞的研究结果之间的差异表明，两性霉素B在完整细胞中参与了复杂的相互作用。