丙泊酚可增强猪和人类红细胞的抗氧化能力。

Propofol enhances red cell antioxidant capacity in swine and humans.

作者信息

Ansley D M, Lee J, Godin D V, Garnett M E, Qayumi A K

机构信息

Department of Anaesthesia, University of British Columbia, Vancouver, Canada.

出版信息

Can J Anaesth. 1998 Mar;45(3):233-9. doi: 10.1007/BF03012908.

DOI:10.1007/BF03012908

PMID:9579261

Abstract

PURPOSE

To determine the effect of an anaesthetic with antioxidant potential, propofol, on red blood cell (RBC) antioxidant enzyme activities and RBC susceptibility to peroxidative challenge.

METHODS

Propofol was administered by intravenous bolus (2.5 mg.kg-1) and continuous infusion (36 and 72 ml.hr-1 in nine swine; 216 ml.hr-1 in two swine), to achieve serum concentrations between 5 and 30 micrograms.ml-1 for two hours at each rate. Arterial blood sampling was at 0, 10, 30, 60, and 120 min for each rate of infusion, for measurement of plasma propofol concentration, activities of plasma and RBC superoxide dismutase, glutathione peroxidase, glutathione reductase, RBC catalase, and RBC malondialdehyde (MDA) formation in response to ex vivo oxidative challenge with t-butyl hydrogen peroxide (tBHP; 1.5 mM). Antioxidant mechanisms were determined by in vitro study of MDA formation, GSH depletion, and oxidation of haemoglobin to methaemoglobin in human erythrocytes exposed to propofol 0-75 microM. The antioxidant potential of propofol was compared with that of alpha-tocopherol utilising the reaction with 2,4,6-tripyridyl-s-triazine (TPTZ).

RESULTS

Propofol had no effect on plasma or RBC antioxidant enzyme activities. It inhibited RBC MDA production over the range of 0-20 micrograms.ml-1 (y = -18.683x + 85.431; R2 = 0.8174). Effective propofol concentrations for 25% and 50% reductions in MDA levels were 7-12 and 12-20 micrograms.ml-1, respectively. Propofol has a similar effect on human erythrocytes in vitro (R2 = 0.98).

CONCLUSION

Propofol antagonises the effects of forced peroxidation of red cells at anaesthetic and sub-anaesthetic concentrations in swine. Its actions include scavenging of oxygen derived free radicals in a tocopherol-like manner.

摘要

目的

确定具有抗氧化潜力的麻醉剂丙泊酚对红细胞（RBC）抗氧化酶活性以及红细胞对过氧化应激易感性的影响。

方法

通过静脉推注（2.5mg·kg⁻¹）和持续输注（9头猪为36和72ml·hr⁻¹；2头猪为216ml·hr⁻¹）给予丙泊酚，以使每种速率下血清浓度在5至30μg·ml⁻¹之间维持2小时。在每种输注速率下，于0、10、30、60和120分钟采集动脉血，用于测量血浆丙泊酚浓度、血浆和红细胞超氧化物歧化酶、谷胱甘肽过氧化物酶、谷胱甘肽还原酶、红细胞过氧化氢酶的活性，以及红细胞丙二醛（MDA）在叔丁基过氧化氢（tBHP；1.5mM）离体氧化应激下的生成情况。通过体外研究丙泊酚（0 - 75μM）对人红细胞中MDA生成、谷胱甘肽（GSH）消耗以及血红蛋白氧化为高铁血红蛋白的影响来确定抗氧化机制。利用丙泊酚与2,4,6 - 三吡啶基 - s - 三嗪（TPTZ）的反应，将丙泊酚的抗氧化潜力与α - 生育酚进行比较。

结果

丙泊酚对血浆或红细胞抗氧化酶活性无影响。在0至20μg·ml⁻¹范围内，它抑制红细胞MDA生成（y = -18.683x + 85.431；R² = 0.8174）。使MDA水平降低25%和50%的有效丙泊酚浓度分别为7至12μg·ml⁻¹和12至20μg·ml⁻¹。丙泊酚在体外对人红细胞有类似作用（R² = 0.98）。