Avramović V, Vlahović P, Savić V, Stanković M
Institute of Histology and Embryology, Faculty of Medicine, Yugoslavia.
ORL J Otorhinolaryngol Relat Spec. 1998 May-Jun;60(3):174-7. doi: 10.1159/000027588.
The histochemical localisation of two ecto-enzymes, 5'-nucleotidase (5'-NT) and Mg(2+)-ATPase, was investigated in hyperplastic and recurrent tonsillitis. Detection of enzymes was performed on frozen sections using the classical lead nitrate method. Activity of 5'-NT was demonstrated particularly in the cells of lymphoid follicles and in the basal layer of the surface tonsillar epithelium. There was no difference in localisation of 5'-NT between hyperplastic and recurrent tonsillitis, whereas a stronger reaction in follicular mantle zones was observed in recurrent tonsillitis compared to hyperplastic tonsillitis. Mg(2+)-ATPase activity was mainly associated with the cells lining the tonsillar crypt, with the interfollicular areas and blood vessels. In recurrent tonsillitis only half of the studied follicular germinal centres expressed Mg(2+)-ATPase activity, compared to hyperplastic tonsillitis. The similar localisation of 5'-NT and ecto-ATPase in both types of chronic tonsillitis suggests that in inflamed tonsils expression of investigated enzymes probably does not depend on the type of chronic tonsillitis.
在增生性和复发性扁桃体炎中研究了两种外切酶，即5'-核苷酸酶（5'-NT）和镁离子-ATP酶的组织化学定位。使用经典的硝酸铅法在冰冻切片上进行酶的检测。5'-NT的活性特别在淋巴滤泡细胞和扁桃体表面上皮的基底层中得到证实。增生性和复发性扁桃体炎之间5'-NT的定位没有差异，而与增生性扁桃体炎相比，在复发性扁桃体炎的滤泡套区观察到更强的反应。镁离子-ATP酶活性主要与扁桃体隐窝内衬细胞、滤泡间区域和血管相关。与增生性扁桃体炎相比，在复发性扁桃体炎中，仅一半的研究滤泡生发中心表达镁离子-ATP酶活性。两种类型的慢性扁桃体炎中5'-NT和外切ATP酶的相似定位表明，在发炎的扁桃体中，所研究酶的表达可能不依赖于慢性扁桃体炎的类型。
