Brandão A, Urmenyi T, Rondinelli E, Gonzalez A, de Miranda A B, Degrave W
Departamento de Bioquímica e Biologia Molecular, Instituto Oswaldo Cruz, Rio de Janeiro, Brasil.
Mem Inst Oswaldo Cruz. 1997 Nov-Dec;92(6):863-6. doi: 10.1590/s0074-02761997000600024.
Random single pass sequencing of cDNA fragments, also known as generation of Expressed Sequence Tags (ESTs), has been highly successful in the study of the gene content of higher organisms, and forms an integral part of most genome projects, with the objective to identify new genes and targets for disease control and prevention and to generate mapping probes. In the Trypanosoma cruzi genome project, EST sequencing has also been a starting point, and here we report data on the first 797 sequences obtained, partly from a CL Brener epimastigote non-normalized library, partly on a normalized library. Only around 30% of the sequences obtained showed similarity with Genbank and dbEST databases, half of which with sequences already reported for T. cruzi.
对互补DNA（cDNA）片段进行随机单通道测序，即所谓的表达序列标签（EST）生成，在高等生物基因含量研究中取得了巨大成功，并且是大多数基因组计划的重要组成部分，目的是识别新基因以及疾病控制与预防的靶点，并生成定位探针。在克氏锥虫基因组计划中，EST测序也是一个起点，在此我们报告首批获得的797个序列的数据，部分来自CL Brener型无鞭毛体非标准化文库，部分来自标准化文库。所获得的序列中只有约30%与Genbank和dbEST数据库中的序列相似，其中一半与已报道的克氏锥虫序列相似。
