Stormo G D, Fields D S
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder 80309-0347, USA.
Trends Biochem Sci. 1998 Mar;23(3):109-13. doi: 10.1016/s0968-0004(98)01187-6.
Site-specific DNA-protein interactions can be studied using experimental and computational methods. Experimental approaches typically analyze a protein-DNA interaction by measuring the free energy of binding under a variety of conditions. Computational methods focus on alignments of known binding sites for a protein, and, from these alignments, make estimates of the binding energy. Understanding the relationship between these two perspectives, and finding ways to improve both, is a major challenge of modern molecular biology.
位点特异性的DNA-蛋白质相互作用可以通过实验和计算方法来研究。实验方法通常通过测量在各种条件下的结合自由能来分析蛋白质-DNA相互作用。计算方法则专注于蛋白质已知结合位点的比对，并从这些比对中估计结合能。理解这两种观点之间的关系，并找到改进两者的方法，是现代分子生物学的一项重大挑战。
