Suzuki M
MRC Laboratory of Molecular Biology, Cambridge, UK.
Protein Eng. 1995 Jan;8(1):1-4. doi: 10.1093/protein/8.1.1.
DNA recognition by a beta-sheet is discussed in the light of crystal structures of the MetJ and Arc repressors. The DNA binding geometry of a beta-sheet can be understood in terms of (i) close fitting of the two surfaces and (ii) matching of residue and base positions. A beta-sheet is not entirely flat but has a curvature. A beta-sheet of the Met-Arc family faces the DNA major groove with its convex surface; the local DNA major groove is deepest at the centre. The beta-sheet follows 6 bp; every two residues face the DNA and the first and fifth residues, which are separated by 13.2 A, bind, respectively, to the third and sixth bases, which are separated by 13.5 A, on the same DNA strand.
根据MetJ和Arc阻遏物的晶体结构，讨论了β-折叠对DNA的识别。β-折叠的DNA结合几何结构可以从以下两方面来理解：（i）两个表面的紧密贴合；（ii）残基与碱基位置的匹配。β-折叠并非完全平坦，而是具有曲率。Met-Arc家族的β-折叠以其凸面朝向DNA大沟；局部DNA大沟在中心处最深。β-折叠沿着6个碱基对排列；每两个残基面向DNA，相距13.2埃的第一个和第五个残基分别与同一条DNA链上相距13.5埃的第三个和第六个碱基结合。
