Shelykh G I, Kol'tsova S V, Vlasov G P, Samsonov G V
Prikl Biokhim Mikrobiol. 1979 Jan-Feb;15(1):82-7.
Trypsin modified by dextran preactivated with 2-amino-4,6-dichloro-1,3,5-triazine or ethyl chloroformate has been prepared. Properties of the resulting conjugates with respect to hydrolysis of synthetic substrates--methyl ester benzoyl L-arginine and n-nitrophenyl ester of n'-guanidine benzoic acid as well as high molecular weight substrates--casein and fibrin--have been studied. Stability of conjugates during autolysis and irreversible heat denaturation has been investigated.
已制备了用2-氨基-4,6-二氯-1,3,5-三嗪或氯甲酸乙酯预活化的葡聚糖修饰的胰蛋白酶。研究了所得缀合物对合成底物——甲基酯苯甲酰-L-精氨酸和N'-胍基苯甲酸的对硝基苯酯以及高分子量底物——酪蛋白和纤维蛋白的水解特性。还研究了缀合物在自溶和不可逆热变性过程中的稳定性。
