双氢睾酮对人增生性腺中前列腺酸性磷酸酶mRNA转录的影响。
The effect of dihydrotestosterone on transcription of prostatic acid phosphatase mRNA in human hyperplastic gland.
作者信息
Dulińska J, Laidler P, Ostrowski W S, Mrozicki S, Gałka M
机构信息
Institute of Medical Biochemistry, Collegium Medicum, Jagiellonian University, Cracow, Poland.
出版信息
Acta Biochim Pol. 1997;44(4):751-8.
The effect of 5alpha-dihydrotestosterone (DHT) on the level of human prostatic acid phosphatase (hPAP) mRNA was studied using tissue slices from various benign prostatic hyperplastic glands. The absence of DHT in the incubation medium led to a gradual, significant decrease of the hPAP mRNA level. Addition of the hormone induced hPAP mRNA in a time- and dose-dependent manner. The maximal 2-4-fold induction by 10(-9) M DHT was observed after 3-5 h of incubation, and then the hPAP mRNA level was 6-20-fold higher than that in a parallel sample incubated without DHT. The results suggest that DHT is necessary to sustain the expression of hPAP in hyperplastic prostates.
利用来自各种良性前列腺增生腺体的组织切片，研究了5α-双氢睾酮（DHT）对人前列腺酸性磷酸酶（hPAP）mRNA水平的影响。孵育培养基中缺乏DHT会导致hPAP mRNA水平逐渐显著下降。添加该激素会以时间和剂量依赖的方式诱导hPAP mRNA。在孵育3-5小时后，观察到10^(-9) M DHT产生最大2-4倍的诱导作用，然后hPAP mRNA水平比未添加DHT孵育的平行样本高6-20倍。结果表明，DHT是维持增生前列腺中hPAP表达所必需的。
Zotero 插件