Lin Y
Zhonghua Yi Xue Za Zhi. 1991 May;71(5):263-5, 20.
To Study the relationship between the activity of 5 alpha-reductase and benign hyperplastic prostate (BPH), we have measured the T and dihydrotestosterone (DHT) contents and the specific activity of this enzyme in mechanically separated stroma and epithelium and in both the nucleus and cytoplasm from 20 cases of BPH and 7 cases of normal human prostate. The results were as follows: (1) The content of DHT in the BPH group was about 3 times higher than that in the N group. (2) The Michaelis constant (Km) of 5 alpha-reductase in both the stroma and epithelium was similar. (3) The specific activity of the enzyme in the stroma was higher than that in the epithelium, and in the BPH group, increased activity was found in the tissue or stroma but not in the epithelium. The activity was predominant in the nucleus. So our experiment supports that the elevation of the activity of 5 alpha-reductase may be related to the pathogenesis of human BPH.
为研究5α-还原酶活性与良性前列腺增生（BPH）之间的关系，我们测定了20例BPH患者和7例正常男性前列腺组织经机械分离得到的基质和上皮组织以及细胞核和细胞质中的睾酮（T）和双氢睾酮（DHT）含量及该酶的比活性。结果如下：（1）BPH组DHT含量约为正常组的3倍。（2）基质和上皮组织中5α-还原酶的米氏常数（Km）相似。（3）该酶在基质中的比活性高于上皮组织，在BPH组中，组织或基质中的酶活性增加，但上皮组织中未增加。酶活性主要存在于细胞核中。因此，我们的实验支持5α-还原酶活性升高可能与人类BPH的发病机制有关。
